中国麻业科学
中國痳業科學
중국마업과학
PLANT FIBER SCIENCES IN CHINA
2014年
5期
226-231
,共6页
温岚%龚友才%陈基权%戴志刚%粟建光
溫嵐%龔友纔%陳基權%戴誌剛%粟建光
온람%공우재%진기권%대지강%속건광
甲基环酸乙酯(EMS)%60Co-γ%黄麻%突变体
甲基環痠乙酯(EMS)%60Co-γ%黃痳%突變體
갑기배산을지(EMS)%60Co-γ%황마%돌변체
methyl acid ethyl ester (EMS)%60Co -γ%jute%mutant
为丰富黄麻种质资源和扩大突变体容量,本研究采用物理诱变(60Co-γ)和化学诱变(EMS)相结合的方法,以“中黄麻4号”等7个黄麻品种为材料,观察记录诱变黄麻苗期的形态学变化,分析诱变黄麻的代谢特性和抗性指标。结果表明EMS和60Co-γ射线对黄麻幼苗的叶片产生不同程度的损伤,前者主要导致叶片卷曲,后者导致叶片分叉。复合诱变导致黄麻幼苗脯氨酸含量、丙二醛含量和根系活力提高。而可溶性蛋白、SOD活性和POD活性变化则品种之间呈现差异。本研究为黄麻种质创新的方法提供了参考,并且丰富了黄麻突变体的材料。
為豐富黃痳種質資源和擴大突變體容量,本研究採用物理誘變(60Co-γ)和化學誘變(EMS)相結閤的方法,以“中黃痳4號”等7箇黃痳品種為材料,觀察記錄誘變黃痳苗期的形態學變化,分析誘變黃痳的代謝特性和抗性指標。結果錶明EMS和60Co-γ射線對黃痳幼苗的葉片產生不同程度的損傷,前者主要導緻葉片捲麯,後者導緻葉片分扠。複閤誘變導緻黃痳幼苗脯氨痠含量、丙二醛含量和根繫活力提高。而可溶性蛋白、SOD活性和POD活性變化則品種之間呈現差異。本研究為黃痳種質創新的方法提供瞭參攷,併且豐富瞭黃痳突變體的材料。
위봉부황마충질자원화확대돌변체용량,본연구채용물리유변(60Co-γ)화화학유변(EMS)상결합적방법,이“중황마4호”등7개황마품충위재료,관찰기록유변황마묘기적형태학변화,분석유변황마적대사특성화항성지표。결과표명EMS화60Co-γ사선대황마유묘적협편산생불동정도적손상,전자주요도치협편권곡,후자도치협편분차。복합유변도치황마유묘포안산함량、병이철함량화근계활력제고。이가용성단백、SOD활성화POD활성변화칙품충지간정현차이。본연구위황마충질창신적방법제공료삼고,병차봉부료황마돌변체적재료。
In order to expand jute germplasm resources and increase mutants capacity ,physi-cal (radiation of 60 Co -γ) and chemical (EMS) means were both employed in this study .More-over ,mutant seedlings of 7 varieties such as “Zhong Huang Ma No.4” were observed and recor-ded ,some metabolic characteristics and resistance parameters w ere also analyzed. T he results showed that ,EMS resolution and 60Co -γ radiation caused different damage on jute leaves ,the former caused leaves crinkled and the latter caused leaves forked. Combined means including EMS and 60Co -γ radiation resulted in the enhancement of praline content 、MDA content and root activity. However ,the variation of soluble protein content 、SOD activity and POD activity were different among varieties. This study might give some references in the innovation of jute germplasm ,and enrich the jute mutant materials.