CAJ | 학술논문

采用L16（45）的正交试验确立了适合三叶木通（Akebia trifoliate）相关序列扩增多态性（SRAP）最佳反应体系为（25μL）：1×Reaction Buffer、Mg2＋2．5 mmol／L、dNTPs0．2 mmol／L、Taq 酶2U、引物0．4μmol／L、DNA 20 ng／25μL．利用最佳体系从100对SRAP引物中筛选出6对，对秦岭地区3个居群的62份三叶木通进行遗传多样性分析，共得到142条条带，多态性比率为87．32％．聚类分析结果显示：在遗传相似系数为0．72时，可将62份三叶木通划分为3个组群，与样品采集地的居群相一致．表明该SRAP体系能够提供丰富的信息位点，适用于三叶木通遗传多样性的分析．
채용L16（45）적정교시험학립료괄합삼협목통（Akebia trifoliate）상관서렬확증다태성（SRAP）최가반응체계위（25μL）：1×Reaction Buffer、Mg2＋2．5 mmol／L、dNTPs0．2 mmol／L、Taq 매2U、인물0．4μmol／L、DNA 20 ng／25μL．이용최가체계종100대SRAP인물중사선출6대，대진령지구3개거군적62빈삼협목통진행유전다양성분석，공득도142조조대，다태성비솔위87．32％．취류분석결과현시：재유전상사계수위0．72시，가장62빈삼협목통화분위3개조군，여양품채집지적거군상일치．표명해SRAP체계능구제공봉부적신식위점，괄용우삼협목통유전다양성적분석．
SRAP-PCR amplification system on Akebia trifoliate was optimized by the orthogonal design of L16 (45 ),The optimal PCR system was obtained as 1 × Reaction Buffer,2.5 mmol/L Mg2+,0.2 mmol/L dNTPs,2 U TaqDNA polymerase,0.4μmol/L primer,20 ng genomic DNA in 25μL reaction system.6 primer pairs from 100 primer combinations were selected to analyze the genetic diversity relationship of 6 2 strains Akebia trifoliate from Qinling region.There were 142 clear bands produced,the rate of polymorphism was 87.32%.The result of cluster analysis based on SRAP markers showed that 62 stains Akebiatrifoliate could be clustered into 3 groups at 0.72 of genetic similarity coefficient.Every individual of one population was clustered in the same group.It is consistant with the sample collecting zone in group.Therefore,the SRAP system can provide abundant information site,which can be used in the research of genetic diversity of Akebia trifoliate in future.