临床精神医学杂志
臨床精神醫學雜誌
림상정신의학잡지
JOURNAL OF CLINICAL PSYCHOLOGICAL MEDICINE
2014年
5期
315-319
,共5页
姚能云%佘艳梅%周红勤%赵锋%唐普照%罗浦花%刘丽桃%戴建垒%徐平
姚能雲%佘豔梅%週紅勤%趙鋒%唐普照%囉浦花%劉麗桃%戴建壘%徐平
요능운%사염매%주홍근%조봉%당보조%라포화%류려도%대건루%서평
精神分裂症%p24 基因片段%p40 基因片段%博尔纳病病毒
精神分裂癥%p24 基因片段%p40 基因片段%博爾納病病毒
정신분렬증%p24 기인편단%p40 기인편단%박이납병병독
schizophrenia%p24 gene%p40 gene%borna disease virus
目的：探讨精神分裂症患者发病与博尔纳病病毒（BDV）感染的关系，分析被 BDV 感染的精神分裂症患者临床特征。方法：用荧光定量巢式逆转录聚合酶链反应（FQ-nRT-PCR）方法检测86例精神分裂症患者（病例组）和84例健康体检者（对照组）外周血单个核细胞（PBMCs）中 BDVp24和 BD-Vp40基因片段，用β-肌动蛋白（β-actin）作为内参照，并总结阳性患者的临床特征，病例组采用阳性与阴性症状量表（PANSS）评分。结果：病例组外周血标本 BDV p24基因片段检出率11.6%（10/86），BDV p40基因片段检出率14.0%（12/86），拷贝数均>102 kb/μl。对照组 BDV p24、BDV p40基因片段均为阴性（0%，0/84），两组阳性率差异有统计学意义（P 均<0.05）。BDV p24和 BDV p40基因片段均为阳性的标本测序后，与 BDV 标准病毒株 V 和马源的 BDV 病毒株 H1766序列比较同源性分别为96.35%和98.85%。在4个位点出现基因突变（nt1649 T→C、nt1656 G→A、nt1670 C→T 和 nt1676 C→T）。该目的基因片段与马源的 BDV 病毒株亲缘关系最近。BDV 阳性患者精神症状主要以幻觉、妄想为主。结论：精神分裂症发病与 BDV 感染有一定的相关性，BDV 阳性患者以阳性精神症状为主要临床特征。
目的：探討精神分裂癥患者髮病與博爾納病病毒（BDV）感染的關繫，分析被 BDV 感染的精神分裂癥患者臨床特徵。方法：用熒光定量巢式逆轉錄聚閤酶鏈反應（FQ-nRT-PCR）方法檢測86例精神分裂癥患者（病例組）和84例健康體檢者（對照組）外週血單箇覈細胞（PBMCs）中 BDVp24和 BD-Vp40基因片段，用β-肌動蛋白（β-actin）作為內參照，併總結暘性患者的臨床特徵，病例組採用暘性與陰性癥狀量錶（PANSS）評分。結果：病例組外週血標本 BDV p24基因片段檢齣率11.6%（10/86），BDV p40基因片段檢齣率14.0%（12/86），拷貝數均>102 kb/μl。對照組 BDV p24、BDV p40基因片段均為陰性（0%，0/84），兩組暘性率差異有統計學意義（P 均<0.05）。BDV p24和 BDV p40基因片段均為暘性的標本測序後，與 BDV 標準病毒株 V 和馬源的 BDV 病毒株 H1766序列比較同源性分彆為96.35%和98.85%。在4箇位點齣現基因突變（nt1649 T→C、nt1656 G→A、nt1670 C→T 和 nt1676 C→T）。該目的基因片段與馬源的 BDV 病毒株親緣關繫最近。BDV 暘性患者精神癥狀主要以幻覺、妄想為主。結論：精神分裂癥髮病與 BDV 感染有一定的相關性，BDV 暘性患者以暘性精神癥狀為主要臨床特徵。
목적：탐토정신분렬증환자발병여박이납병병독（BDV）감염적관계，분석피 BDV 감염적정신분렬증환자림상특정。방법：용형광정량소식역전록취합매련반응（FQ-nRT-PCR）방법검측86례정신분렬증환자（병례조）화84례건강체검자（대조조）외주혈단개핵세포（PBMCs）중 BDVp24화 BD-Vp40기인편단，용β-기동단백（β-actin）작위내삼조，병총결양성환자적림상특정，병례조채용양성여음성증상량표（PANSS）평분。결과：병례조외주혈표본 BDV p24기인편단검출솔11.6%（10/86），BDV p40기인편단검출솔14.0%（12/86），고패수균>102 kb/μl。대조조 BDV p24、BDV p40기인편단균위음성（0%，0/84），량조양성솔차이유통계학의의（P 균<0.05）。BDV p24화 BDV p40기인편단균위양성적표본측서후，여 BDV 표준병독주 V 화마원적 BDV 병독주 H1766서렬비교동원성분별위96.35%화98.85%。재4개위점출현기인돌변（nt1649 T→C、nt1656 G→A、nt1670 C→T 화 nt1676 C→T）。해목적기인편단여마원적 BDV 병독주친연관계최근。BDV 양성환자정신증상주요이환각、망상위주。결론：정신분렬증발병여 BDV 감염유일정적상관성，BDV 양성환자이양성정신증상위주요림상특정。
Objective:To explore the relationship between the patients with schizophrenia and Borna dis-ease virus(BDV)infection as well as analyze the clinical features of patients infected by BDV. Method:The p24 and p40 genes fragment of BDV in patients with 86 schizophrenic patients(case group)and 84 healthy con-trols(control group)were examined in peripheral blood mononuclear cells(PBMCs)by nested with fluorescence quantitative nested real-time polymerase chain reaction(FQ-nRT-PCR). β-actin in specimens were detected by PCR as internal reference. The clinical characteristics of BDVp24 or BDVp40 positive patients were summa-rized. Positive and negative syndrome scale(PANSS)was used to evaluate case group. Results:The positive rate(10 / 86,11. 6% )and(12 / 86,14. 0% )of BDV p24 and p40 in PBMCs in case group were significantly higher than that in control group(0 / 84,0% )(all P < 0. 05),and the number of copies of BDV p24 and p40 in PBMCs were more than 102 kb/ μl. The homology of the gene sequence of positive product of PBMCs were 96. 35% and 98. 85% ,which had 4 situs of consistent silent mutation(nt1649 T→C,nt1656 G→A,nt1670 C→T, nt1676 C→T),compared with the sequence of the standard strain V and the virus strain H1766 of BDV from horse supplied by GenBank. The homology of the gene sequence of positive product was genetically relative to the virus strain BDV from horse. The clinical features in BDV positive patients with schizophrenia were mainly hallucination and delusion. Conclusion:BDV infection and schizophrenia may have certain relations. The clinical features in BDV positive patients with schizophrenia are mainly positive symptoms.
