CAJ | 학술논문

目的：探讨1型糖尿病（T1DM）小鼠胰岛表达胰岛淀粉样多肽（IAPP）、生长抑素（SS）的改变及可能的机制。方法取正常C57BL／6J小鼠，小剂量多次注射链脲菌素（STZ）建立1型糖尿病小鼠模型，分别于第3、7、10、14、21、28天取胰尾组织，通过免疫组织化学SABC法、激光扫描共焦显微镜免疫荧光检测法及图像分析、形态计量法进行研究。结果1．IAPP阳性细胞面数密度（NA）自第3天开始减少（P＜0．05），平均吸光度自第7天开始增高，与正常及生理盐水对照组比较差异具有显著性（ P＜0．05）；2．1型糖尿病组小鼠胰岛SS阳性细胞NA自第3天开始增加（P＜0．05），平均吸光度自第7天开始增高（P＜0．05）；3．免疫荧光双染法显示，小鼠胰岛IAPP和SS在部分细胞有共表达。结论1型糖尿病小鼠胰岛IAPP阳性细胞数目减少，表达增强；SS阳性细胞数量及表达均上调；IAPP和SS在部分细胞有共表达。提示IAPP阳性细胞和SS阳性细胞均参与了1型糖尿病的病变过程。
목적：탐토1형당뇨병（T1DM）소서이도표체이도정분양다태（IAPP）、생장억소（SS）적개변급가능적궤제。방법취정상C57BL／6J소서，소제량다차주사련뇨균소（STZ）건립1형당뇨병소서모형，분별우제3、7、10、14、21、28천취이미조직，통과면역조직화학SABC법、격광소묘공초현미경면역형광검측법급도상분석、형태계량법진행연구。결과1．IAPP양성세포면수밀도（NA）자제3천개시감소（P＜0．05），평균흡광도자제7천개시증고，여정상급생리염수대조조비교차이구유현저성（ P＜0．05）；2．1형당뇨병조소서이도SS양성세포NA자제3천개시증가（P＜0．05），평균흡광도자제7천개시증고（P＜0．05）；3．면역형광쌍염법현시，소서이도IAPP화SS재부분세포유공표체。결론1형당뇨병소서이도IAPP양성세포수목감소，표체증강；SS양성세포수량급표체균상조；IAPP화SS재부분세포유공표체。제시IAPP양성세포화SS양성세포균삼여료1형당뇨병적병변과정。
Objective To explore the changes of the expression of islet amylodi polypeptide ( IAPP ) and somatostatin( SS) of islet in type 1 diabetes mice, and the mechanism of the expression changes .Methods We established the diabetes model in C57BL/6J mice by low-dose streptozotocin ( STZ) injection.The excisions of the pancreas tails removed on the 3th, 7th, 10th, 14th, 21th and 28th day.Tissues were assessed by immunohistochemical SABC, immunofluorescence in the study .Results 1.Numerical density on area ( NA ) of IAPP positive cells in experimental group (EG) decreased since the 3th day, but average absorbance increased since the 7th day.2.NA of SS-IR cells in EG increased since the 3th day, and average absorbance increased since the 7th day.3.The results of immunofluorescence double staining showed that , IAPP and SS could coexpression in part of cells in islets .Conclusion The number of IAPP positive cells in type 1 diabetes is decreased , but the immunoreaction increased .Immunoreaction and number of SS positive cells increase .Both of them are involved in the pathogenesis of type 1 diabetes.