CAJ | 학술논문

目的：筛选合并代谢异常的帕金森病模型，为后续的相关实验研究提供理想的动物模型。方法SD雄性大鼠62只，随机分为损毁前脑内侧束（ MFB）模型组、损毁MFB假手术组、损毁纹状体模型组和损毁纹状体假手术组。模型制做成功后进行旋转实验，两组大鼠中分别选出成功模型，并分别对两组成功的PD模型大鼠的摄食、体重、血糖和腹腔内脂肪组织进行检测。观察两组模型大鼠上述代谢指标的变化。结果损毁MFB较损毁纹状体制作帕金森病模型的成功率高（70．97％比38．71％）。与各假手术组大鼠相比，MFB模型大鼠既出现体重明显的减轻[（218．1±13．99）g比（252．7±10．1）g， P＜0．05]，腹腔注射葡萄糖后15min和30min血糖出现了明显升高，并且肾周白色脂肪组织明显减少（左右两侧）。纹状体模型大鼠仅饮食减少[（13．95±0．25） g 比（20．23±0．86）g， P＜0．001]和腹腔注射葡萄糖后15min、30min和60min时间点血糖升高。结论损毁MFB的PD模型大鼠无论模型成功率还是代谢相关指标都较损毁纹状体的PD模型更接近糖脂代谢异常的PD患者。
목적：사선합병대사이상적파금삼병모형，위후속적상관실험연구제공이상적동물모형。방법SD웅성대서62지，수궤분위손훼전뇌내측속（ MFB）모형조、손훼MFB가수술조、손훼문상체모형조화손훼문상체가수술조。모형제주성공후진행선전실험，량조대서중분별선출성공모형，병분별대량조성공적PD모형대서적섭식、체중、혈당화복강내지방조직진행검측。관찰량조모형대서상술대사지표적변화。결과손훼MFB교손훼문상체제작파금삼병모형적성공솔고（70．97％비38．71％）。여각가수술조대서상비，MFB모형대서기출현체중명현적감경[（218．1±13．99）g비（252．7±10．1）g， P＜0．05]，복강주사포도당후15min화30min혈당출현료명현승고，병차신주백색지방조직명현감소（좌우량측）。문상체모형대서부음식감소[（13．95±0．25） g 비（20．23±0．86）g， P＜0．001]화복강주사포도당후15min、30min화60min시간점혈당승고。결론손훼MFB적PD모형대서무론모형성공솔환시대사상관지표도교손훼문상체적PD모형경접근당지대사이상적PD환자。
Objective To select an ideal Parkinson ’ s disease ( PD) animal model with metabolic abnormalities for subsequent experimental studies .Methods A total of 62 Sprague-Dawley male rats were randomly divided into four groups:damaged medial forebrain bundle ( MFB) model group, damaged medial forebrain bundle ( MFB) sham group, damaged Striatum model group and damaged Striatum sham group .After detecting the rotation experiment , successful model rats of two groups were selected to detect the changes of food intake , body weight , blood glucose and intra-abdominal adipose tissue.Results It was easier to produce a PD model by destroying MFB than striatum .Compared with sham-operated rats, MFB model rats showed significant abnormality both in reduction of body weight [(218.1 ±13.99) g vs (252.7 ±10.1)g, P<0.05] and high blood glucose appeared at 15min and 30min after introperitoneal glucose tolerance test ( IPGTT) .Their perirenal white adipose tissue was significantly reduced ( both left and right side ) .Striatum model rats only appeared decreased food intake [(13.95 ±0.25)g vs (20.23 ±0.86)g, P<0.001] and impaired glucose regulation at 15min, 30min and 60min after IPGTT.Their body weight and adipose tissue did not change significantly .Conclusion No matter in the success rate or metabolism-related indicators , MFB damaged rat model of PD is more suitable to study PD patients with abnormal lipid metabolism compared with Striatum rat model .