CAJ | 학술논문

目的：检测钠尿肽受体（ NPR）在不同年龄小鼠视网膜内的表达，探讨其在视网膜发育过程中的作用。方法收集从受孕16日（E16）到出生90日（P90）小鼠眼球标本共127只，对NPR-A进行免疫荧光检测。结果 NPR-A广泛存在于视网膜神经元中，例如，在外核层，NPR-A于P7开始高表达在视锥、视杆细胞内、外突起上，于P14减弱，P30之后持续稳定弱表达；在内核层，从P7开始NPR-A持续弱表达在双极细胞的突起中，而在水平细胞中未见NPR-A表达；在神经节细胞层，NPR-A于E16开始高表达在神经节细胞胞体中，P14明显减弱，而在神经纤维层，即神经节细胞的轴突中，NPR-A从胚胎期至成年持续高表达；在外网状层和内网状层，NPR-A于P14均高表达，但于P30之后逐渐减弱。此外，NPR-A还广泛的存在于Müller细胞的突起中。结论 NPR-A参与了视网膜的发育，可能是小鼠视网膜神经元发育过程中的关键分子，并对Müller细胞的功能活动起着重要的调节作用。
목적：검측납뇨태수체（ NPR）재불동년령소서시망막내적표체，탐토기재시망막발육과정중적작용。방법수집종수잉16일（E16）도출생90일（P90）소서안구표본공127지，대NPR-A진행면역형광검측。결과 NPR-A엄범존재우시망막신경원중，례여，재외핵층，NPR-A우P7개시고표체재시추、시간세포내、외돌기상，우P14감약，P30지후지속은정약표체；재내핵층，종P7개시NPR-A지속약표체재쌍겁세포적돌기중，이재수평세포중미견NPR-A표체；재신경절세포층，NPR-A우E16개시고표체재신경절세포포체중，P14명현감약，이재신경섬유층，즉신경절세포적축돌중，NPR-A종배태기지성년지속고표체；재외망상층화내망상층，NPR-A우P14균고표체，단우P30지후축점감약。차외，NPR-A환엄범적존재우Müller세포적돌기중。결론 NPR-A삼여료시망막적발육，가능시소서시망막신경원발육과정중적관건분자，병대Müller세포적공능활동기착중요적조절작용。
Objective Our purpose is to investigate the expression of natriuretic peptide receptor A (NPR-A) in the retina and to understand the NPR-A’ s functions during the mouse development .Methods Mice eyes were harvested from E16 ( embryonic day 16 ) to P90 ( postnatal day 90 ) . Total of 127 eyes were used in the study . Immunohistochemistries of NPR-A were carried out .Results During development , NPR-A was widely expressed in the retinal neurons .In the outer nuclear layer , NPR-A began to appear in the inner and outer projections of cone and rod cells at P7, but decreased at P14.From P30 afterward, it continued to express weakly .In the inner nuclear layer , NPR-A expressed in the dendrites of bipolar cells weakly from P 7 to adulthood , whereas no expression in horizontal cells .In the ganglion cell layer, NPR-A started highly to express in the ganglion cell bodies at E 16, and in the meantime, in the nerve fiber layer , ganglion cell axons , NPR-A was expressed highly from embryonic to adult .In the inner and outer plexiform layers, NPR-A was highly expressed at P14, but decreased gradually after P30.In addition, NPR-A also widely expressed in the inner protrusions of Müller cells.Conclusion NPR-A participates in the development of the retina , and may be the key molecule in the developing retinal neurons .Moreover, it plays an important regulatory role in the functional activity of Müller cells .