CAJ | 학술논문

金磁纳米微粒因兼具独特的光学性质及超顺磁性而受到广泛关注。对这种复合材料表面标记蛋白的定量为后续应用提供了必要的前提，也为免疫探针的构建提供了空间分布的基本信息。鉴于此，本研究对金磁纳米微粒表面偶联蛋白进行定量。表面改性的金磁纳米微粒表面含有大量羧基，为后续的蛋白偶联提供了偶联位点，以碳二亚胺（EDC ）作为连接因子可将链亲合素（SA ）化学键合于金磁纳米微粒表面。为得到准确的蛋白偶联率，以三氯乙酸排除EDC对Low ry法的干扰，结合金磁纳米微粒密度的计算以及SA 的相对分子量，可以确定其偶联率为18个S A／金磁微粒。
금자납미미립인겸구독특적광학성질급초순자성이수도엄범관주。대저충복합재료표면표기단백적정량위후속응용제공료필요적전제，야위면역탐침적구건제공료공간분포적기본신식。감우차，본연구대금자납미미립표면우련단백진행정량。표면개성적금자납미미립표면함유대량최기，위후속적단백우련제공료우련위점，이탄이아알（EDC ）작위련접인자가장련친합소（SA ）화학건합우금자납미미립표면。위득도준학적단백우련솔，이삼록을산배제EDC대Low ry법적간우，결합금자납미미립밀도적계산이급SA 적상대분자량，가이학정기우련솔위18개S A／금자미립。
Gold magnetic nanoparticles have attracted considerable interest in biomedicine fields due to their unique optical properties and super paramagnetism .T he determination of protein content provide prerequisite for further applications and necessary messages to inves-tigate the micro-construction of immunoassay probe based on gold magnetic nanoparticle ,so we studied coupling efficiency of streptavidin (SA) immobilizing on the composite nanoparti-cle′s surface in this paper .In order to achieve the greatest coupling efficiency ,particles were firstly coated by polymers to get plenty carboxyl groups on the surface ,then 1-(3-dimethyl-laminopropyl)-3-ethyl carbodiimide hydrochloride(EDC)was explored as a linker to achieve chemical bond .During the process of protein determination ,trichloroacetic acid was used to remove the interference of EDC in Lowry method .At last the coupling efficiency about 18 SA/nanoparticle was achieved by calculations based on composite nanoparticle′s density and streptavidin′s relative molecular mass .