茶叶科学
茶葉科學
다협과학
2014年
5期
435-441
,共7页
李亚莉%康冠宏%杨丽源%祝红昆%李治滢%周红杰
李亞莉%康冠宏%楊麗源%祝紅昆%李治瀅%週紅傑
리아리%강관굉%양려원%축홍곤%리치형%주홍걸
普洱茶%黄曲霉%黄曲霉毒素%LC-MS/MS
普洱茶%黃麯黴%黃麯黴毒素%LC-MS/MS
보이다%황곡매%황곡매독소%LC-MS/MS
Pu′er tea%Aspergillus flavus%aflatoxins%LC-MS/MS
以云南大叶种晒青茶为原料,接种产毒黄曲霉进行模拟普洱茶发酵试验,并在发酵结束时通过LC-MS/MS 法对茶样进行黄曲霉毒素检测,研究普洱茶发酵过程中黄曲霉的生长及产毒情况。结果表明,在普洱茶发酵过程中,接种的黄曲霉能在茶叶中生长繁殖,初期生长较快,但随发酵时间的延长,黄曲霉在茶叶中的生长明显受到抑制,其数量逐渐下降。发酵终止时,未在茶样中检测出黄曲霉毒素。
以雲南大葉種曬青茶為原料,接種產毒黃麯黴進行模擬普洱茶髮酵試驗,併在髮酵結束時通過LC-MS/MS 法對茶樣進行黃麯黴毒素檢測,研究普洱茶髮酵過程中黃麯黴的生長及產毒情況。結果錶明,在普洱茶髮酵過程中,接種的黃麯黴能在茶葉中生長繁殖,初期生長較快,但隨髮酵時間的延長,黃麯黴在茶葉中的生長明顯受到抑製,其數量逐漸下降。髮酵終止時,未在茶樣中檢測齣黃麯黴毒素。
이운남대협충쇄청다위원료,접충산독황곡매진행모의보이다발효시험,병재발효결속시통과LC-MS/MS 법대다양진행황곡매독소검측,연구보이다발효과정중황곡매적생장급산독정황。결과표명,재보이다발효과정중,접충적황곡매능재다협중생장번식,초기생장교쾌,단수발효시간적연장,황곡매재다협중적생장명현수도억제,기수량축점하강。발효종지시,미재다양중검측출황곡매독소。
For the purpose of studying the Aspergillus flavus growth and toxin production in Pu′er tea fermentation, the Yunnan large-leaves solar-drying green tea was used as the raw material to fermentate by inoculating toxigenic Aspergillus flavus fungus in Pu′er tea. Aflatoxin was tested by LC-MS/MS method at the end of tea samples fermentation. The results showed that during the tea fermentation, Aspergillus flavus growth was observed in the initial fermentation. However, with the extension of Pu′er tea fermentation time, Aspergillus flavus growth were inhibited in the late fermentation, the number decreased gradually, while the end of fermentation, the aflatoxin was not detected in tea samples.