CAJ | 학술논문

为研究低蛋白质日粮添加赖氨酸和蛋氨酸对仔鹿氮代谢的影响，采用4×4完全拉丁方试验，选取3月龄、健康、离乳梅花鹿仔鹿4只，体重为（30±0．12）kg（-X±SD），进行消化代谢试验。对照组（Ⅰ组）饲喂16．28％粗蛋白质的高蛋白质日粮，试验组饲喂13．40％粗蛋白质，添加0．23％赖氨酸，并分别添加0（Ⅱ组）、0．06％（Ⅲ组）、0．12％（Ⅳ组）蛋氨酸的低蛋白质日粮。结果表明：1）对照组蛋白质消化率显著高于Ⅱ组（P＜0．01）、Ⅲ组和Ⅳ组（P＜0．05），脂肪消化率显著低于Ⅱ组（P＜0．01），其他营养物质消化率差异不显著（P＞0．05）。2）对照组和Ⅳ组蛋氨酸消化率显著高于Ⅱ组和Ⅲ组（P＜0．05），对照组其余氨基酸消化率（赖氨酸、胱氨酸除外）显著高于各试验组（P＜0．05）。3）对照组吸收氮显著高于Ⅱ组（P＜0．01）、Ⅲ组和Ⅳ组（P＜0．05），尿氮排放量显著高于各试验组（P＜0．05），氮利用率及氮生物学效价低于各试验组（P＞0．05）。4）对照组尿嘌呤衍生物总排放量显著大于Ⅱ组（P＜0．01）和Ⅲ组（P＜0．05），尿囊素显著大于Ⅱ组（P＜0．01）、Ⅲ组和Ⅳ组（P＜0．05），对照组、Ⅲ组和Ⅳ组黄嘌呤和次黄嘌呤显著高于Ⅱ组（P＜0．01）。补充赖氨酸、蛋氨酸可改善低蛋白质日粮仔鹿营养物质消化率利用率，除粗蛋白外其他营养物质消化率与常规蛋白质水平相近，甚至更好，提高氮沉积、氮利用率和生物学效价，改善氮平衡，减少氮排放，降低环境污染。
위연구저단백질일량첨가뢰안산화단안산대자록담대사적영향，채용4×4완전랍정방시험，선취3월령、건강、리유매화록자록4지，체중위（30±0．12）kg（-X±SD），진행소화대사시험。대조조（Ⅰ조）사위16．28％조단백질적고단백질일량，시험조사위13．40％조단백질，첨가0．23％뢰안산，병분별첨가0（Ⅱ조）、0．06％（Ⅲ조）、0．12％（Ⅳ조）단안산적저단백질일량。결과표명：1）대조조단백질소화솔현저고우Ⅱ조（P＜0．01）、Ⅲ조화Ⅳ조（P＜0．05），지방소화솔현저저우Ⅱ조（P＜0．01），기타영양물질소화솔차이불현저（P＞0．05）。2）대조조화Ⅳ조단안산소화솔현저고우Ⅱ조화Ⅲ조（P＜0．05），대조조기여안기산소화솔（뢰안산、광안산제외）현저고우각시험조（P＜0．05）。3）대조조흡수담현저고우Ⅱ조（P＜0．01）、Ⅲ조화Ⅳ조（P＜0．05），뇨담배방량현저고우각시험조（P＜0．05），담이용솔급담생물학효개저우각시험조（P＞0．05）。4）대조조뇨표령연생물총배방량현저대우Ⅱ조（P＜0．01）화Ⅲ조（P＜0．05），뇨낭소현저대우Ⅱ조（P＜0．01）、Ⅲ조화Ⅳ조（P＜0．05），대조조、Ⅲ조화Ⅳ조황표령화차황표령현저고우Ⅱ조（P＜0．01）。보충뢰안산、단안산가개선저단백질일량자록영양물질소화솔이용솔，제조단백외기타영양물질소화솔여상규단백질수평상근，심지경호，제고담침적、담이용솔화생물학효개，개선담평형，감소담배방，강저배경오염。
The effect of a low crude protein (CP)diet supplemented with amino acids on nitrogen metabolism in four three month old sika deer fawns [mean live weight (30±0.12)kg]was investigated using a 4×4 Latin square design experiment.Four diets were compared;control (groupⅠ)fed a 16.28% crude protein diet,a group fed 13.40 % CP diet supplemented with 0.23% lysine and 0 (group Ⅱ),0.06% (group Ⅲ),0.12%(group Ⅳ)methionine.The results show that the CP digestibility of the control group was significantly greater than group Ⅱ (P<0.01),Ⅲ and Ⅳ (P<0.05)but ether extract digestibility was lower than group Ⅱ (P<0.01).No treatment differences for other nutrients were found (P>0.05).Methionine digestibility in the con-trol group and group Ⅳ was significantly greater than group Ⅱ and group Ⅲ (P<0.05);digestibility of other amino acids (except lysine and cystine)in the control group was significantly greater than all other groups (P<0.05).Nitrogen (N)absorption in the control group was significantly greater than group Ⅱ (P<0.01),Ⅲand Ⅳ (P<0.05)while urine N emissions were significantly greater and N utilization lower than other groups (P<0.05).Total urine purine emissions in the control group were significantly greater than group Ⅱ (P<0.01)and Ⅲ (P<0.05)and allantoin emissions significantly greater than group Ⅱ (P<0.01),Ⅲ and Ⅳ(P<0.05).Xanthine and hypoxanthine emissions in group Ⅱ was significantly lower than the control group (P<0.01),groups Ⅲ and Ⅳ (P<0.05).Supplementing with lysine and methionine improved the apparent digestibility of a low CP diet,approaching the digestibility of diets with a normal protein content and improving N retention and N utilization,reducing N emissions and helping to protect the environment.