CAJ | 학술논문

通过测定分离自箭薚豌豆和玉米根际4株细菌的固氮酶活性、溶磷量及分泌生长素能力，将其制成植物根际接种剂，并结合半固体培养试验测定接种剂对箭薚豌豆生长的影响。结果表明，菌株J3-1、J1-15和Y16具备溶磷和分泌生长素能力，J1-15的溶磷能力最强，为548．9 mg／L，Y16分泌生长素能力最好，达17．8μg／mL，且菌株Y16具较强固氮能力，J3固氮酶活性为366．51 C2 H4 nmol／（mL·h）。与对照组相比，单一菌株制备的接种剂处理（Y16）可使箭薚豌豆地上生物量、地下生物量分别显著增加104．5％和254．1％（P＜0．05），复合接种剂处理F（J3-1＋J1-15＋Y16＋J3）使箭薚豌豆地上和地下生物量分别增加76．1％和192．3％。综合各指标，复合接种剂处理效果明显优于单一接种剂，处理F（J3-1＋J1-15＋Y16＋J3）可使箭薚豌豆株高、根长、根表面积、根体积、根系活力，分别较对照增加29．4％，70．0％，174．0％，194．6％，38．3％。这主要是由于菌种间的互作效应造成的。
통과측정분리자전탕완두화옥미근제4주세균적고담매활성、용린량급분비생장소능력，장기제성식물근제접충제，병결합반고체배양시험측정접충제대전탕완두생장적영향。결과표명，균주J3-1、J1-15화Y16구비용린화분비생장소능력，J1-15적용린능력최강，위548．9 mg／L，Y16분비생장소능력최호，체17．8μg／mL，차균주Y16구교강고담능력，J3고담매활성위366．51 C2 H4 nmol／（mL·h）。여대조조상비，단일균주제비적접충제처리（Y16）가사전탕완두지상생물량、지하생물량분별현저증가104．5％화254．1％（P＜0．05），복합접충제처리F（J3-1＋J1-15＋Y16＋J3）사전탕완두지상화지하생물량분별증가76．1％화192．3％。종합각지표，복합접충제처리효과명현우우단일접충제，처리F（J3-1＋J1-15＋Y16＋J3）가사전탕완두주고、근장、근표면적、근체적、근계활력，분별교대조증가29．4％，70．0％，174．0％，194．6％，38．3％。저주요시유우균충간적호작효응조성적。
To investigate the effect of rhizobacteria inoculants on the growth ofViciasativa,four rhizobacteria strains (J3-1,J1-15,Y16 and J3)were assessed for potentially useful characteristics with the potential to en-hance the growth and yield ofV.sativa;high nitrogenase activity,high phosphate solubilisation and ability to secrete 3-indoleacetic acid (IAA)into the rhizosphere.Strains J3-1,J1-15 and Y16 had the ability to solubilize phosphate and secrete IAA.J1-15,Y16,J3-1 had the strongest phosphate solubilizing capacity (548.9 mg/L), IAA secreting ability (17.8μg/mL),and nitrogenase activity (366.51 C2 H4 nmol/mL·h),respectively.Y16 increased the aboveground biomass and underground biomass ofV.sativaby 104.5%and 254.1%,respective-ly,compared to the non-inoculated control.An inoculant mixture of all four strains increased the aboveground biomass and underground biomass ofV.sativaby 76.1% and 192.3%,respectively,relative to the control. This resulted from an increase in plant height,root length,root surface area,root volume and root activity of 29.4%,70.0%,174.0%,194.6% and 38.3%,respectively.The results suggest that inoculants including mixtures of rhizobacteria strains may have significant benefits forV.sativacultivation.