草业学报
草業學報
초업학보
PRATACULTURAL SCIENCE
2014年
5期
193-200
,共8页
时振振%李胜%杨柯%马绍英%刘会杰%张品南%杨晓明
時振振%李勝%楊柯%馬紹英%劉會傑%張品南%楊曉明
시진진%리성%양가%마소영%류회걸%장품남%양효명
NO%响应%豌豆幼苗
NO%響應%豌豆幼苗
NO%향응%완두유묘
NO%response%pea seedling
以豌豆品种“陇豌一号”为材料,用NO 供体硝普钠、NO 清除剂 c-PTIO 及硝普钠类似物亚铁氰化钠处理豌豆幼苗,研究了内外源NO 对盐胁迫下豌豆幼苗生长的影响。结果表明:添加外源 NO 能明显使盐胁迫下豌豆幼苗胚芽和胚根长、幼苗干重、根冠比及叶绿素含量增加(P<0.05),可溶性蛋白和可溶性糖含量升高,豌豆幼苗叶片SOD、POD和CAT 活性增加(P<0.05),叶片质膜相对透性降低,还能减缓 MDA含量的上升并提高脯氨酸含量。内源NO 对盐胁迫下豌豆幼苗胚芽生长、幼苗干重、可溶性糖含量、MDA 含量以及SOD、CAT 活性均具有明显的调节作用。因此,内外源NO 均可缓解盐胁迫对豌豆幼苗的伤害。
以豌豆品種“隴豌一號”為材料,用NO 供體硝普鈉、NO 清除劑 c-PTIO 及硝普鈉類似物亞鐵氰化鈉處理豌豆幼苗,研究瞭內外源NO 對鹽脅迫下豌豆幼苗生長的影響。結果錶明:添加外源 NO 能明顯使鹽脅迫下豌豆幼苗胚芽和胚根長、幼苗榦重、根冠比及葉綠素含量增加(P<0.05),可溶性蛋白和可溶性糖含量升高,豌豆幼苗葉片SOD、POD和CAT 活性增加(P<0.05),葉片質膜相對透性降低,還能減緩 MDA含量的上升併提高脯氨痠含量。內源NO 對鹽脅迫下豌豆幼苗胚芽生長、幼苗榦重、可溶性糖含量、MDA 含量以及SOD、CAT 活性均具有明顯的調節作用。因此,內外源NO 均可緩解鹽脅迫對豌豆幼苗的傷害。
이완두품충“롱완일호”위재료,용NO 공체초보납、NO 청제제 c-PTIO 급초보납유사물아철청화납처리완두유묘,연구료내외원NO 대염협박하완두유묘생장적영향。결과표명:첨가외원 NO 능명현사염협박하완두유묘배아화배근장、유묘간중、근관비급협록소함량증가(P<0.05),가용성단백화가용성당함량승고,완두유묘협편SOD、POD화CAT 활성증가(P<0.05),협편질막상대투성강저,환능감완 MDA함량적상승병제고포안산함량。내원NO 대염협박하완두유묘배아생장、유묘간중、가용성당함량、MDA 함량이급SOD、CAT 활성균구유명현적조절작용。인차,내외원NO 균가완해염협박대완두유묘적상해。
A pea variety ‘longwan1 ’was used as the experimental material to study the effect of endogenous and exogenous NO on the growth of pea seedlings under NaCl stress with NO donor sodium nitroprusside (SNP),NO scavenger c-PTIO and sodium nitroprusside analogues sodium ferrocyanide.It was found that adding exogenous NO increased the plumule and radicle length,seedling dry weight,root-shoot ratio,chloro-phyll content,soluble protein and sugar levels,and the activities of SOD,POD and CAT of the pea seedlings. The content of proline was also increased under salt stress.Meanwhile,exogenous NO also alleviated the accu-mulation of MDA and decreased the membrane relative permeability in leaves of pea seedlings under salt stress. Endogenous NO had a significant effect on growth of germ,seedling dry weight,soluble sugar content,and MDA,SOD and CAT activity.In summary,exogenous and endogenous NO both alleviated the damage to pea seedlings caused by salt stress.