CAJ | 학술논문

以“拉丁诺”（Ladino）白三叶为供试材料，研究NaCl对PEG（聚乙二醇6000）水分胁迫下白三叶幼苗叶片相对含水量、根系活力、叶绿素含量、活性氧成分、膜相对透性和膜脂过氧化、抗氧化防御及有机渗透调节物质积累的影响。外源NaCl能显著提高水分胁迫下叶片相对含水量和根系活力，有效降低叶绿素的分解；通过增强水分胁迫下叶片SOD、POD和CAT活性及抗坏血酸-谷胱甘肽循环（AsA-GSH cycle）关键酶活性，维持了细胞内显著较低的活性氧、电解质渗透率和MDA含量，缓解了胁迫对细胞造成的氧化性伤害；50 mmol／L NaCl对正常水分条件下白三叶的生长也具有一定的促进作用；但外源NaCl预处理使水分胁迫下白三叶叶片内可溶性糖和游离脯氨酸含量显著降低。说明NaCl显著提高白三叶的抗旱性与增强胁迫下白三叶抗氧化防御系统密切相关，并非促进了植物体内有机渗透调节物质的积累，而可能像其他旱生植物一样是通过富集Na＋提高其渗透调节能力来发挥作用。
이“랍정낙”（Ladino）백삼협위공시재료，연구NaCl대PEG（취을이순6000）수분협박하백삼협유묘협편상대함수량、근계활력、협록소함량、활성양성분、막상대투성화막지과양화、항양화방어급유궤삼투조절물질적루적영향。외원NaCl능현저제고수분협박하협편상대함수량화근계활력，유효강저협록소적분해；통과증강수분협박하협편SOD、POD화CAT활성급항배혈산-곡광감태순배（AsA-GSH cycle）관건매활성，유지료세포내현저교저적활성양、전해질삼투솔화MDA함량，완해료협박대세포조성적양화성상해；50 mmol／L NaCl대정상수분조건하백삼협적생장야구유일정적촉진작용；단외원NaCl예처리사수분협박하백삼협협편내가용성당화유리포안산함량현저강저。설명NaCl현저제고백삼협적항한성여증강협박하백삼협항양화방어계통밀절상관，병비촉진료식물체내유궤삼투조절물질적적루，이가능상기타한생식물일양시통과부집Na＋제고기삼투조절능력래발휘작용。
Effects of NaCl on relative water content,root viability,chlorophyll content,reactive oxygen spe-cies,relative membrane permeability and membrane lipid peroxidation,antioxidant defence and accumulation of osmoregulatory substances in white clover (Trifoliumrepens)under water stress induced by PEG-6000 were analyzed.Under water stress,exogenous NaCl significantly improved relative water content and root viability, and effectively reduced the decomposition of chlorophyll in leaves.The treatment of exogenous NaCl improved SOD,POD,CAT activities and the key enzymes of AsA-GSH cycle activities and maintained significantly low-er electrolyte leakage and MDA content,thereby reducing water deficit stress injury to plants.50 mmol/L NaCl also can improve the growth of white clover under normal water availability,but the accumulation of sol-uble sugar and free proline was significantly lower in the exogenous NaCl treatment under water deficit stress. The results suggested that exogenous NaCl can effectively improve water deficit stress tolerance of white clover with mechanisms including enhanced antioxidant defence,but not accumulation of more osmoregulatory sub-stances.The reason maybe that white clover is like other xerophytes which accumulate Na+ as an osmoregula-tory solute under drought stress.