草业学报
草業學報
초업학보
PRATACULTURAL SCIENCE
2014年
5期
168-174
,共7页
宋辉%南志标%蔡小宁%钟小仙%顾洪如
宋輝%南誌標%蔡小寧%鐘小仙%顧洪如
송휘%남지표%채소저%종소선%고홍여
海滨雀稗%液泡膜 H+-PPase%RACE%耐盐
海濱雀稗%液泡膜 H+-PPase%RACE%耐鹽
해빈작패%액포막 H+-PPase%RACE%내염
Paspalumvaginatum%H+-PPase%RACE%salt-tolerance
根据已公布液泡膜 H＋-PPase基因家族同源序列保守区设计简并引物，克隆出海滨雀稗中PvVP1基因的中间序列，然后用快速扩增cDNA末端（rapid amplification of cDNA end，RACE）方法，从海滨雀稗中克隆到PvVP15′cDNA序列。该序列ORF长1605 bp，编码535个氨基酸，其编码序列、氨基酸序列与玉米相应基因的同源性分别为93％和99％。
根據已公佈液泡膜 H＋-PPase基因傢族同源序列保守區設計簡併引物，剋隆齣海濱雀稗中PvVP1基因的中間序列，然後用快速擴增cDNA末耑（rapid amplification of cDNA end，RACE）方法，從海濱雀稗中剋隆到PvVP15′cDNA序列。該序列ORF長1605 bp，編碼535箇氨基痠，其編碼序列、氨基痠序列與玉米相應基因的同源性分彆為93％和99％。
근거이공포액포막 H＋-PPase기인가족동원서렬보수구설계간병인물，극륭출해빈작패중PvVP1기인적중간서렬，연후용쾌속확증cDNA말단（rapid amplification of cDNA end，RACE）방법，종해빈작패중극륭도PvVP15′cDNA서렬。해서렬ORF장1605 bp，편마535개안기산，기편마서렬、안기산서렬여옥미상응기인적동원성분별위93％화99％。
A pair of degenerated primers were designed based on previously cloned homologous cDNA seg-ments.The middle segment of PvVP1 was cloned,then the PvVP1 5 ’cDNA sequence also obtained using 5 ’ RACE.The open reading frame (ORF)of this sequence was 1605 bp,encoding 534 amino acids.The codon and amino acid sequences of the 5’ends of thePvVP1 gene shared 93% and 99% similarity,respectively,with those of Zeamays.
