草业学报
草業學報
초업학보
PRATACULTURAL SCIENCE
2014年
5期
161-167
,共7页
Ta6-SFT%果聚糖%油菜%干旱胁迫
Ta6-SFT%果聚糖%油菜%榦旱脅迫
Ta6-SFT%과취당%유채%간한협박
Ta6-SFT%fructan%Brassicanapus%drought stress
为了研究Ta6-SFT对油菜抗旱的影响，进行了Ta6-SFT对油菜纯系材料的遗传转化，获得经PCR、South-ern杂交和Northern 斑点杂交验证的转基因植株。对7株T1代转基因油菜进行了为期36 d的干旱胁迫，分析干旱胁迫36 d和复水后2 d各植株及野生型对照中的Ta6-SFT的转录水平表达和果聚糖含量，同时进行同时期丙二醛含量和相对电导率测定。结果表明，Ta6-SFT的转录水平表达与转基因植株体内的果聚糖含量、转基因植株的抗旱性呈正相关，表明Ta6-SFT在干旱胁迫下的表达增强了转基因植株的抗旱性。
為瞭研究Ta6-SFT對油菜抗旱的影響，進行瞭Ta6-SFT對油菜純繫材料的遺傳轉化，穫得經PCR、South-ern雜交和Northern 斑點雜交驗證的轉基因植株。對7株T1代轉基因油菜進行瞭為期36 d的榦旱脅迫，分析榦旱脅迫36 d和複水後2 d各植株及野生型對照中的Ta6-SFT的轉錄水平錶達和果聚糖含量，同時進行同時期丙二醛含量和相對電導率測定。結果錶明，Ta6-SFT的轉錄水平錶達與轉基因植株體內的果聚糖含量、轉基因植株的抗旱性呈正相關，錶明Ta6-SFT在榦旱脅迫下的錶達增彊瞭轉基因植株的抗旱性。
위료연구Ta6-SFT대유채항한적영향，진행료Ta6-SFT대유채순계재료적유전전화，획득경PCR、South-ern잡교화Northern 반점잡교험증적전기인식주。대7주T1대전기인유채진행료위기36 d적간한협박，분석간한협박36 d화복수후2 d각식주급야생형대조중적Ta6-SFT적전록수평표체화과취당함량，동시진행동시기병이철함량화상대전도솔측정。결과표명，Ta6-SFT적전록수평표체여전기인식주체내적과취당함량、전기인식주적항한성정정상관，표명Ta6-SFT재간한협박하적표체증강료전기인식주적항한성。
Fructan is thought to be one of the important regulators involved in plant tolerance to various abiotic stresses.In this study the Triticum aestivum gene for sucrose:fructan 6-fructosyltransferase synthesis (Ta6-SFT)was transferred into Brassicanapus,driven by the rd29A promoter and mediated by Agrobacteriumtu-mefaciens.Subsequently,transgenic plants were identified using PCR,southern blot and northern dot blot tests.In order to test the effect of theTa6-SFTgene on transgenic plants under drought stress,non-transgen-ic plants (control)and 7 transgenic plants (T1 generation)were exposed to 36 days of moisture stress.Expres-sion of Ta6-SFT was tested using a semi-quantitative RT-PCR 3 6 days after treatment and 2 days after re-watering.Fructan concentration and physiological traits including relative conductivity and MDA content were analyzed at the same times.The results showed Ta6-SFT expression level is positively associated with fructan content and resistance to drought stress,indicating that the Ta6-SFT gene could enhance resistance to drought in transgenic plants.
