CAJ | 학술논문

目的：检测干细胞标记物LGR5（富含亮氨酸重复单位的G蛋白偶联受体5）蛋白在人结直肠上皮内瘤变中的表达，探讨与其临床病理特征的关系。方法采用免疫组织化学染色法，分别检测60例结直肠上皮内瘤变（腺瘤并高级别上皮内瘤变32例，腺瘤并低级别上皮内瘤变28例）、10例腺瘤，20例高分化腺癌标本中LGR5表达水平。结果LGR5在高分化腺癌中表达率为100．00％（20／20），明显高于结直肠上皮内瘤变[83．33％（50／60）]和腺瘤[40．00％（4／10）]，P＜0．01。高级别上皮内瘤变组和低级别上皮内瘤变组中其阳性表达率分别为84．37％（27／32）、82．14％（23／28），LGR5表达水平与结直肠上皮内瘤变病理分级无相关性（P＞0．05）。结论 LGR5可作为选择结直肠上皮内瘤变手术方式的重要参考指标。
목적：검측간세포표기물LGR5（부함량안산중복단위적G단백우련수체5）단백재인결직장상피내류변중적표체，탐토여기림상병리특정적관계。방법채용면역조직화학염색법，분별검측60례결직장상피내류변（선류병고급별상피내류변32례，선류병저급별상피내류변28례）、10례선류，20례고분화선암표본중LGR5표체수평。결과LGR5재고분화선암중표체솔위100．00％（20／20），명현고우결직장상피내류변[83．33％（50／60）]화선류[40．00％（4／10）]，P＜0．01。고급별상피내류변조화저급별상피내류변조중기양성표체솔분별위84．37％（27／32）、82．14％（23／28），LGR5표체수평여결직장상피내류변병리분급무상관성（P＞0．05）。결론 LGR5가작위선택결직장상피내류변수술방식적중요삼고지표。
Objective To investigate the expression of stem cell marker LGR 5( rich in leucine repeated units of G pro-tein coupled receptor 5) protein in colorectal intraepithelial and its relationships with clinicopathologic characteristics .Methods LGR5 expression was immunohistochemically determined on paraffin-embedded ,formalin-fixed biopsy tissues in 60 cases of color-ectal intraepithelial neoplasia (32 cases of adenoma and high-grade intraepithelial neoplasia ,28 cases of adenoma and low level in-traepithelial neoplasia ) ,10 cases of adenoma and 20 cases of well-differentiated adenocarcinoma .Results The expression rate of LGR5 in well-differentiated adenocarcinoma was 100.00%(20/20),significantly higher than 83.33%(50/60)in colorectal in-traepithelial neoplasia adenoma and 40.00%(4/10)in colorectal adenoma (P<0.01).The expression rates of LGR5 in the high level and low level intraepithelial neoplasia were 84.37%(27/32)and 82.14%(23/28)respectively,there are no correlation be-tween LGR5 expression level and intraepithelial neoplasia pathologic stage (P>0.05).Conclusion LGR5 may be considered as an important reference index in selsecting operation method for colorectal intraepithelial neoplasia .