CAJ | 학술논문

目的：探讨早期胃癌中原代细胞ssDNA适配子检测的临床意义。方法对5个标志物应用适配子-生物素-链霉亲和素磁珠分离系统进行分离、纯化及鉴定，筛选出特异性、灵敏度高的肿瘤标志物，并对其建立相应的检测方法，采用美国临床实验室标准化组织的相关标准对其检测方法进行临床评价，并参考《临床检验方法学评价》对其诊断性能进行分析。结果早期胃癌中原代细胞ssDNA适配子的检测敏感性为49．13％，特异性为91．44％，阳性似然比为5．74，阴性似然比为0．56 g，干扰实验51．8％，验前比65．7％，验后比197．2％。与金标准相比，在敏感性、特异性、准确度和精确度方面均无明显差异（P＞0．05）。结论早期胃癌中原代细胞ssDNA适配子的检测，具有较高的灵敏度和较强的特异性，为早期胃癌的发现提供依据，可考虑作为早期胃癌的辅助诊断标准之一。
목적：탐토조기위암중원대세포ssDNA괄배자검측적림상의의。방법대5개표지물응용괄배자-생물소-련매친화소자주분리계통진행분리、순화급감정，사선출특이성、령민도고적종류표지물，병대기건립상응적검측방법，채용미국림상실험실표준화조직적상관표준대기검측방법진행림상평개，병삼고《림상검험방법학평개》대기진단성능진행분석。결과조기위암중원대세포ssDNA괄배자적검측민감성위49．13％，특이성위91．44％，양성사연비위5．74，음성사연비위0．56 g，간우실험51．8％，험전비65．7％，험후비197．2％。여금표준상비，재민감성、특이성、준학도화정학도방면균무명현차이（P＞0．05）。결론조기위암중원대세포ssDNA괄배자적검측，구유교고적령민도화교강적특이성，위조기위암적발현제공의거，가고필작위조기위암적보조진단표준지일。
Objective To analyze the clinical significance of detection of primary cell of ssDNA aptamer in early gastric adenocarcinoma .Methods The tumor markers of specificity and high sensitivity were selected by the method of separation ,puri-fication and identification based on the 5 markers using the established aptamer-mildew avidin biotin-chain magnetic bead ,and set up a corresponding test method ,used the clinical laboratory standardization organization's standards on its detection methods for clinical evaluation ,and referred to the clinical test methodology to evaluate and analyze the diagnostic performance .Results Sen-sitivity of the primitive cells ssDNA aptamer in early gastric cancer was 49.13%,specificity was 91.44%,positive likelihood ratio was 5.74,negative likelihood ratio was 0.56 g,interference experiment was 51.8%,pre-test odds was 65.7%,and post-test odds was 197.2%.Compared with the gold standard ,there were no obvious difference in sensitivity ,specificity,accuracy and precision (P>0.05).Conclusion The detection of primitive cells ssDNA aptamer in early gastric cancer has high sensitivity and strong specificity,it provides the basis for detection of early gastric cancer ,and can be considered as one of the auxiliary diagnostic crite-ria of early gastric cancer .