实用癌症杂志
實用癌癥雜誌
실용암증잡지
THE PRACTICAL JOURNAL OF CANCER
2014年
9期
1067-1069,1081
,共4页
MCM2%乳腺癌%细胞增殖
MCM2%乳腺癌%細胞增殖
MCM2%유선암%세포증식
Minichromosome maintenance protein 2%Breast cancer%Proliferation
目的：探讨微型染色体维持蛋白2（ minichromosome maintenance proteins 2，MCM2）基因和蛋白水平在乳腺癌组织中的表达变化及其临床意义。方法应用RT-PCR技术，半定量检测在54例乳腺癌组织、12例乳腺良性疾病组织和10例正常乳腺组织中MCM2 mRNA的表达水平。应用Western-blotting技术检测MCM2蛋白在上述组织中的表达变化。结果正常乳腺组织、良性和恶性乳腺肿瘤组织中MCM2 mRNA和蛋白表达水平比较存在统计学差异（ P＜0．05），且随着肿瘤恶性程度的升高呈逐渐增高趋势。 MCM2 mRNA和蛋白表达量在不同TNM分期和不同病理类型乳腺癌组织间存在统计学差异（P＜0．05），但Ⅰ期与Ⅱ期、Ⅲ期与Ⅳ期间MCM2表达却无统计学差异（P＞0．05）。结论MCM2可以用于判断乳腺癌的恶性程度和病理分级的高低，可能为乳腺癌的未来基因治疗提供一个新的潜在分子靶点和理论依据。
目的：探討微型染色體維持蛋白2（ minichromosome maintenance proteins 2，MCM2）基因和蛋白水平在乳腺癌組織中的錶達變化及其臨床意義。方法應用RT-PCR技術，半定量檢測在54例乳腺癌組織、12例乳腺良性疾病組織和10例正常乳腺組織中MCM2 mRNA的錶達水平。應用Western-blotting技術檢測MCM2蛋白在上述組織中的錶達變化。結果正常乳腺組織、良性和噁性乳腺腫瘤組織中MCM2 mRNA和蛋白錶達水平比較存在統計學差異（ P＜0．05），且隨著腫瘤噁性程度的升高呈逐漸增高趨勢。 MCM2 mRNA和蛋白錶達量在不同TNM分期和不同病理類型乳腺癌組織間存在統計學差異（P＜0．05），但Ⅰ期與Ⅱ期、Ⅲ期與Ⅳ期間MCM2錶達卻無統計學差異（P＞0．05）。結論MCM2可以用于判斷乳腺癌的噁性程度和病理分級的高低，可能為乳腺癌的未來基因治療提供一箇新的潛在分子靶點和理論依據。
목적：탐토미형염색체유지단백2（ minichromosome maintenance proteins 2，MCM2）기인화단백수평재유선암조직중적표체변화급기림상의의。방법응용RT-PCR기술，반정량검측재54례유선암조직、12례유선량성질병조직화10례정상유선조직중MCM2 mRNA적표체수평。응용Western-blotting기술검측MCM2단백재상술조직중적표체변화。결과정상유선조직、량성화악성유선종류조직중MCM2 mRNA화단백표체수평비교존재통계학차이（ P＜0．05），차수착종류악성정도적승고정축점증고추세。 MCM2 mRNA화단백표체량재불동TNM분기화불동병리류형유선암조직간존재통계학차이（P＜0．05），단Ⅰ기여Ⅱ기、Ⅲ기여Ⅳ기간MCM2표체각무통계학차이（P＞0．05）。결론MCM2가이용우판단유선암적악성정도화병리분급적고저，가능위유선암적미래기인치료제공일개신적잠재분자파점화이론의거。
Objective To investigate the expression of minichromosome maintenance protein 2 ( MCM2 ) mRNA and protein in breast cancer and its clinical significance .Methods Reverse transcription polymerase chain reaction (RT-PCR) was used in semi quantitative detection of the expression of MCM 2 mRNA in 54 cases of breast cancer ,12 cases of benign breast tumor,and 10 cases of normal breast tissues .The expression of MCM2 protein in the above tissues was detected by Western-blot-ting.Results The expression of MCM2 mRNA and protein were significantly different among normal breast ,benign breast tumor and breast cancer tissues .The higher degree of malignancy breast cancer showed ,the higher expression of MCM 2 mRNA and pro-tein were (P<0.05).The expression of MCM2 mRNA and protein showed a statistically significant association with TNM staging and histological grade (P<0.05).There was no significant difference in the expression of MCM 2 between stage ⅠandⅡbreast cancer (P>0.05).The similar result was found between stage Ⅲand Ⅳ breast cancer (P>0.05).Conclusion MCM2 may serve as a reliable and useful diagnostic marker for breast cancer and can evaluate the degree of malignancy and differentiation in breast cancer .MCM2 may serve as a new potential biomarker and theoretic basis in the gene therapy for breast cancer .
