CAJ | 학술논문

[目的]研究铜藻多糖的水提法提取工艺,并研究其抗氧化活性.[方法]采用水提法提取铜藻多糖,研究提取时间、提取温度和料液比等因素对多糖提取率的影响,以多糖得率为指标,通过正交试验优化最佳提取工艺;并对铜藻多糖的1,1-二苯基苦基苯肼(DP-PH)自由基、超氧阴离子自由基(O2-)的清除能力及抗脂质过氧化能力进行研究.[结果]该多糖水提法提取的最优条件为料液比1∶20g/ml、时间3h、温度60℃,在此条件下,铜藻多糖的提取率为4.9％;铜藻多糖抗氧化活性随着浓度增加而增强,其超氧阴离子(O2-)和DPPH自由基清除能力、抗脂质过氧化能力分别为48.1％、38.8％、34.2％.[结论]该研究优化了铜藻多糖的提取工艺,提取的活性多糖具有一定抗氧化活性,试验优化的工艺稳定可行,为铜藻多糖的后续研究和开发提供依据.
[목적]연구동조다당적수제법제취공예,병연구기항양화활성.[방법]채용수제법제취동조다당,연구제취시간、제취온도화료액비등인소대다당제취솔적영향,이다당득솔위지표,통과정교시험우화최가제취공예;병대동조다당적1,1-이분기고기분정(DP-PH)자유기、초양음리자자유기(O2-)적청제능력급항지질과양화능력진행연구.[결과]해다당수제법제취적최우조건위료액비1∶20g/ml、시간3h、온도60℃,재차조건하,동조다당적제취솔위4.9％;동조다당항양화활성수착농도증가이증강,기초양음리자(O2-)화DPPH자유기청제능력、항지질과양화능력분별위48.1％、38.8％、34.2％.[결론]해연구우화료동조다당적제취공예,제취적활성다당구유일정항양화활성,시험우화적공예은정가행,위동조다당적후속연구화개발제공의거.
[Objective] Extraction technology of polysaccharide from Sargassum herneri (Turn) C.Ag.and its antioxidative activity were studied.[Method] The extraction technology was optimized by orthogonal experiment with the content of polysaccharide which measured by phenol-sulfuric acid method and with the ratio of raw materials to solvent,extracting temperature,extracting time as factors,moreover,1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical,scavenging capacity of superoxide anion radical,anti-lipid peroxidation and reducing power of Sargassum herneri polysaccharide were determined.[Result] The optimal extraction technology of polysaccharide was as follows:the extraction time was 3 h,the extraction temperature was 60 ℃,the ratio of raw material to solvent was 1 ∶ 20 g/ml.The antioxidative activity Sargassum herneri polysaccharide was increased in a concentration-dependent manner,which the scavenging capacity of the DPPH free radical was 38.8％,scavenging capacity of superoxide anion radical was 34.2％ and the capacity of anti-lipid peroxidation was 48.1％,respectively.[Conclusion] The optimal extraction parameters was gained in this study and antioxidative activity of Sargassum herneri polysaccharide were investigated,which provided support for the development of Sargassum herneri polysaccharide.