安徽农业科学
安徽農業科學
안휘농업과학
JOURNAL OF ANHUI AGRICULTURAL SCIENCES
2014年
29期
10097-10098
,共2页
巩养仓%张兴平%邢朝柱%王洪%吴建勇%郭立平%彭凡嘉
鞏養倉%張興平%邢朝柱%王洪%吳建勇%郭立平%彭凡嘉
공양창%장흥평%형조주%왕홍%오건용%곽립평%팽범가
棉花%线粒体RNA提取%方法
棉花%線粒體RNA提取%方法
면화%선립체RNA제취%방법
Cotton%mtRNA extraction%Method
[目的]获得高质量的棉花细胞质雄性不育系线粒体RNA.[方法]以哈克尼西棉细胞质雄性不育系黄化苗为研究对象,对棉花线粒体RNA的提取方法进行探讨.[结果]采取先分离出棉花线粒体再提取RNA的方法,最终获得了质量较好的线粒体RNA(mtRNA).[结论]成功获得了质量较高的mtRNA.
[目的]穫得高質量的棉花細胞質雄性不育繫線粒體RNA.[方法]以哈剋尼西棉細胞質雄性不育繫黃化苗為研究對象,對棉花線粒體RNA的提取方法進行探討.[結果]採取先分離齣棉花線粒體再提取RNA的方法,最終穫得瞭質量較好的線粒體RNA(mtRNA).[結論]成功穫得瞭質量較高的mtRNA.
[목적]획득고질량적면화세포질웅성불육계선립체RNA.[방법]이합극니서면세포질웅성불육계황화묘위연구대상,대면화선립체RNA적제취방법진행탐토.[결과]채취선분리출면화선립체재제취RNA적방법,최종획득료질량교호적선립체RNA(mtRNA).[결론]성공획득료질량교고적mtRNA.
[Objective] The aim was to obtain high quality mitochondrial RNA isolation of cytoplasmic male sterility in cotton.[Method] Taking CMS line of G.harknessii as material,a method of mitochondrial RNA isolation was constructed.[Result]The protocol was that cotton mitochondron was isolated firstly and then mtRNA was extracted using an improved CTAB method.And finally the higher quality mtRNA was obtained.[Conclusion] The study successfully obtain higher quality mtRNA.
