CAJ | 학술논문

目的：探讨Wnt-1、β-catenin蛋白在子宫颈鳞状细胞癌( cervical squamous cell carcinoma, CSCC)中的表达及其与侵袭转移的关系。方法采用免疫组化EliVision法检测78例CSCC组织和30例正常子宫颈组织中Wnt-1、β-catenin蛋白表达。结果在正常子宫颈组织中,Wnt-1、β-catenin蛋白的阳性率分别为20.0%和10.0%；CSCC组织中,Wnt-1、β-catenin蛋白的阳性率分别为56.4%和74.4%,二组差异有统计学意义(P<0.001)。33例CSCC淋巴结转移中Wnt-1、β-catenin蛋白的阳性率分别为72.7%和90.9%；45例CSCC无淋巴结转移中Wnt-1、β-catenin蛋白的阳性率分别为44.4%和62.2%。 Wnt-1、β-catenin蛋白表达与CSCC的分化程度( P <0.05)、浸润深度及 FIGO 分期均有关( P <0.05),与患者年龄、肿瘤大小等均无关。Spearman相关性分析显示：Wnt-1与β-catenin蛋白表达呈正相关关系(rs =0.490,P<0.001)。结论 Wnt-1、β-catenin蛋白的表达异常可能参与CSCC的发生、发展及转移,Wnt-1、β-catenin蛋白均可作为预测CSCC侵袭、转移的潜能及临床预后的指标。
목적：탐토Wnt-1、β-catenin단백재자궁경린상세포암( cervical squamous cell carcinoma, CSCC)중적표체급기여침습전이적관계。방법채용면역조화EliVision법검측78례CSCC조직화30례정상자궁경조직중Wnt-1、β-catenin단백표체。결과재정상자궁경조직중,Wnt-1、β-catenin단백적양성솔분별위20.0%화10.0%；CSCC조직중,Wnt-1、β-catenin단백적양성솔분별위56.4%화74.4%,이조차이유통계학의의(P<0.001)。33례CSCC림파결전이중Wnt-1、β-catenin단백적양성솔분별위72.7%화90.9%；45례CSCC무림파결전이중Wnt-1、β-catenin단백적양성솔분별위44.4%화62.2%。 Wnt-1、β-catenin단백표체여CSCC적분화정도( P <0.05)、침윤심도급 FIGO 분기균유관( P <0.05),여환자년령、종류대소등균무관。Spearman상관성분석현시：Wnt-1여β-catenin단백표체정정상관관계(rs =0.490,P<0.001)。결론 Wnt-1、β-catenin단백적표체이상가능삼여CSCC적발생、발전급전이,Wnt-1、β-catenin단백균가작위예측CSCC침습、전이적잠능급림상예후적지표。
Purpose To investigate the expression of Wnt-1 and β-catenin protein in cervical squamous cell carcinomas ( CSCC) and their relationship with invasion and lymph node metastasis. Methods Expression of Wnt-1 and β-catenin protein were examined on immunohistochemistry containing 78 specimens of CSCC and 30 specimens of normal cervical tissues. Results The positive rates of Wnt-1 and β-catenin protein in normal cervical tissues were 20.0% and 10.0% respectively. The positive rates of Wnt-1 andβ-cate-nin protein in CSCC were 56.4%, and 74.4% respectively. There was a significant difference between the two groups (P<0.001). The positive rates of Wnt-1 andβ-catenin protein in 33 cases with lymph node metastasis of CSCC were 72.7% and 90.9% respective-ly. The positive rates of Wnt-1 andβ-catenin protein in 45 cases with no lymph node metastasis of CSCC were 44.4% and 62.2% re-spectively. The expression of Wnt-1 andβ-catenin protein was significantly related with grades of tumors and depth of invasion and FI-GO stages (P<0.05), and there was no significantly difference between the expression of Wnt-1 andβ-catenin protein and CSCC pa-tients age (P>0.05). Spearman analysis showed that the expression of Wnt-1 protein was positive related to the expression ofβ-cate-nin protein (rs =0.490, P<0.001). Conclusion The abnormal expression of Wnt-1 and β-catenin may be involved in initiation, development, invasion, and metastasis of CSCC, and it is suggested that Wnt-1 and β-catenin be considered as potential markers for invasion, metastasis, and prognosis.