CAJ | 학술논문

目的：探讨mTOR在乳腺癌中的表达及其抑制剂CCI-779对乳腺癌细胞MDA-MB-231增殖及凋亡的影响。方法采用免疫组化SP法检测mTOR蛋白在乳腺癌组织和乳腺癌细胞MDA-MB-231中的表达；MTT法检测mTOR抑制剂CCI-779对MDA-MB-231细胞增殖的影响；应用AnnexinV-FITC/PI法检测CCI-779对MDA-MB-231细胞凋亡的影响。结果 mTOR蛋白在71例乳腺癌组织中的阳性率为54.9%，明显高于32例癌旁组织(阳性率为21.9%)；MDA-MB-231细胞中亦存在mTOR蛋白的表达；mTOR抑制剂CCI-779对MDA-MB-231细胞的增殖具有显著抑制作用，呈浓度和时间依赖性；但CCI-779并不能诱导MDA-MB-231细胞发生凋亡。结论 mTOR与乳腺癌关系密切，其抑制剂CCI-779具有较强的抗MDA-MB-231细胞活性，有望成为乳腺癌治疗的前景药物。
목적：탐토mTOR재유선암중적표체급기억제제CCI-779대유선암세포MDA-MB-231증식급조망적영향。방법채용면역조화SP법검측mTOR단백재유선암조직화유선암세포MDA-MB-231중적표체；MTT법검측mTOR억제제CCI-779대MDA-MB-231세포증식적영향；응용AnnexinV-FITC/PI법검측CCI-779대MDA-MB-231세포조망적영향。결과 mTOR단백재71례유선암조직중적양성솔위54.9%，명현고우32례암방조직(양성솔위21.9%)；MDA-MB-231세포중역존재mTOR단백적표체；mTOR억제제CCI-779대MDA-MB-231세포적증식구유현저억제작용，정농도화시간의뢰성；단CCI-779병불능유도MDA-MB-231세포발생조망。결론 mTOR여유선암관계밀절，기억제제CCI-779구유교강적항MDA-MB-231세포활성，유망성위유선암치료적전경약물。
Purpose To investigate the expression of mTOR in breast cancer, and to observe the effect of CCI-779 on proliferation and apoptosis of MDA-MB-231 cell. Methods Immunohistochemical staining was used to detect the expression of mTOR protein in breast cancer tissue and MDA-MB-231 cell. MTT method was used to show effect of CCI-779 on proliferation of MDA-MB-231 cell. Annex-inV-FITC/PI method was used to show effect of CCI-779 on apoptosis of MDA-MB-231 cell. Results 54.9% in 71 cases of breast cancer tissue could express mTOR protein, the expression was significantly higher than in 32 cases of normal tissue (21.9%), mTOR protein was also detected in MDA-MB-231 cell, CCI-779 could inhibit the proliferation of MDA-MB-231 cell, and show a dose-and time-dependent, but CCI-779 could not induce apoptosis of MDA-MB-231 with AnnexinV-FITC/PI assay. Conclusion mTOR is closely related to the formation of breast cancer, CCI-779 has strong activity against MDA-MB-231 cell, it has prospect for treatment of breast cancer in the future.