CAJ | 학술논문

目的：探讨乳腺原发癌和相应淋巴结转移癌干细胞Wnt、Notch信号通路分子β-catenin、Cyclin D1和Notch1 mRNA表达及意义。方法选取乳腺浸润性导管癌30例，且均伴有淋巴结转移，制备乳腺癌单细胞悬液，通过免疫磁珠分选技术分别提取乳腺原发癌和相应淋巴结转移癌干细胞，采用实时荧光定量PCR技术检测两种癌干细胞Wnt、Notch信号通路关键分子β-catenin、Cyclin D1和Notch1 mRNA的表达。结果乳腺原发癌干细胞和相应淋巴结转移癌干细胞中β-catenin mRNA的表达差异无统计学意义(P>0.05)；淋巴结转移癌干细胞中Cyclin D1和Notch1 mRNA的表达显著高于乳腺原发癌干细胞(P<0.01)。结论与乳腺原发癌干细胞相比，淋巴结转移癌干细胞中Cyclin D1和Notch1处于更高的活化状态，具有更高的侵袭和转移能力，有可能成为乳腺癌的新治疗靶点。
목적：탐토유선원발암화상응림파결전이암간세포Wnt、Notch신호통로분자β-catenin、Cyclin D1화Notch1 mRNA표체급의의。방법선취유선침윤성도관암30례，차균반유림파결전이，제비유선암단세포현액，통과면역자주분선기술분별제취유선원발암화상응림파결전이암간세포，채용실시형광정량PCR기술검측량충암간세포Wnt、Notch신호통로관건분자β-catenin、Cyclin D1화Notch1 mRNA적표체。결과유선원발암간세포화상응림파결전이암간세포중β-catenin mRNA적표체차이무통계학의의(P>0.05)；림파결전이암간세포중Cyclin D1화Notch1 mRNA적표체현저고우유선원발암간세포(P<0.01)。결론여유선원발암간세포상비，림파결전이암간세포중Cyclin D1화Notch1처우경고적활화상태，구유경고적침습화전이능력，유가능성위유선암적신치료파점。
Purpose To investigate the gene expression and significance ofβ-catenin, Cyclin D1 and Notch1 in primary breast cancer stem cells ( BCSC) and matched lymph node metastasis stem cells. Methods 30 cases of breast invasive ductal carcinoma and matched metastasis lymph nodes were made into single cell suspensions, then BCSC were separated from them by immunomagnetic sor-ting. β-catenin, Cyclin D1 and Notch1 gene expressions of Wnt, Notch signaling pathway were detected by real time PCR. ResultsThe expression of β-catenin in primary BCSC and matched lymph nodes metastasis stem cells had statistically no differences ( P >0.05), while the expression of Cyclin D1 and Notch1 in matched lymph nodes metastasis stem cells were significantly higher than the expression in primary BCSC (P<0.01, respectively). Conclusion Compared with the primary cancer stem cells, Cyclin D1 and Notch1 activation in metastasis cancer stem cells are in higher level, which leades to a higher capability of invasion and metastasis, which may be a new therapeutic target.