CAJ | 학술논문

目的：探讨乳腺癌中蛋白激酶C灼(protein kinase C灼, PKC灼)、基质金属蛋白酶-2( matrix metalloproteinase-2, MMP-2)、基质金属蛋白酶-9(matrix metalloproteinase-9, MMP-9)的表达及三者与其侵袭、转移的关系。方法应用免疫组化PV 9000两步法检测PKC灼、MMP-2、MMP-9在100例乳腺癌组织及对应癌旁正常乳腺组织中的表达,并分析三者表达的相关性。应用RNAi技术将合成的PKC灼-siRNA转染入乳腺癌细胞株MDA-MB-231中,即siPKC灼/MDA-MB-231细胞组,以转染空载质粒的scr/MDA-MB-231的细胞组作为对照组,应用Transwell侵袭实验检测细胞的体外侵袭能力。利用Western blot法检测转染细胞中PKC灼蛋白的表达。酶联免疫吸附实验( enzyme-linked immunosorbent assay, ELISA)检测培养基中MMP-2、MMP-9的含量。结果 PKC灼、MMP-2、MMP-9在乳腺浸润性导管癌中的阳性率分别为62.5%、37.5%、32.5%,明显高于乳腺导管内癌和癌旁正常乳腺组织(P均<0.05)。 PKC灼蛋白表达与淋巴结转移、远处转移有关(P均<0.01),与患者年龄、肿瘤大小、临床分期、ER、PR等无关( P均>0.05)；转染 siRNA的 siPKC灼/MDA-MB-231细胞组与 scr/MDA-MB-231组相比,体外侵袭能力以及PKC灼蛋白的表达水平明显下降(P均<0.05),培养基中MMP-2、MMP-9蛋白表达量亦明显下降(P均<0.05)。结论 PKC灼通过影响乳腺癌细胞中MMP-2、MMP-9的分泌来促进乳腺癌的侵袭、转移。
목적：탐토유선암중단백격매C작(protein kinase C작, PKC작)、기질금속단백매-2( matrix metalloproteinase-2, MMP-2)、기질금속단백매-9(matrix metalloproteinase-9, MMP-9)적표체급삼자여기침습、전이적관계。방법응용면역조화PV 9000량보법검측PKC작、MMP-2、MMP-9재100례유선암조직급대응암방정상유선조직중적표체,병분석삼자표체적상관성。응용RNAi기술장합성적PKC작-siRNA전염입유선암세포주MDA-MB-231중,즉siPKC작/MDA-MB-231세포조,이전염공재질립적scr/MDA-MB-231적세포조작위대조조,응용Transwell침습실험검측세포적체외침습능력。이용Western blot법검측전염세포중PKC작단백적표체。매련면역흡부실험( enzyme-linked immunosorbent assay, ELISA)검측배양기중MMP-2、MMP-9적함량。결과 PKC작、MMP-2、MMP-9재유선침윤성도관암중적양성솔분별위62.5%、37.5%、32.5%,명현고우유선도관내암화암방정상유선조직(P균<0.05)。 PKC작단백표체여림파결전이、원처전이유관(P균<0.01),여환자년령、종류대소、림상분기、ER、PR등무관( P균>0.05)；전염 siRNA적 siPKC작/MDA-MB-231세포조여 scr/MDA-MB-231조상비,체외침습능력이급PKC작단백적표체수평명현하강(P균<0.05),배양기중MMP-2、MMP-9단백표체량역명현하강(P균<0.05)。결론 PKC작통과영향유선암세포중MMP-2、MMP-9적분비래촉진유선암적침습、전이。
Purpose To investigate the expressions of PKCζ, MMP-2, and MMP-9 in breast cancer and the relationship with the inva-sion and metastasis of breast cancer. Methods The expression of PKCζ, MMP-2 and MMP-9 in 100 cases with breast cancer was as-sessed with immunohistochemistry PV 9000 method. PKCζ-siRNA was transfected into MDA-MB-231 cell lines, called siPKCζ/MDA-MB-231. While siRNA construct containing a scrambled sequence was transfected into MDA-MB-231 cells to generate control cells, which were designated as Scr/MDA231 cells. Western blotting was used to measure the expression of PKCζ in transfected cells, and the Transwell invasion assay was used to detect the invasion ability in vitro. The content of MMP-2, MMP-9 were measured by ELISA. Results The expression rates of PKCζ, MMP-2 and MMP-9 in breast cancer tissues were 62.5%, 37.5% and 32.5%, and there were significant differences among them (P<0.05). The expression of PKCζwas much higher than those in the normal breast tissues nearby. The expression of PKC protein was assoiated with lymph node metastasis, distant metastasis (P<0.01), but was not correla-ted with other clinicopathologic parameters, such as age, tumor size, histological type, ER, PR, and so on (P>0.05). The expres-sion of PKCζ, MMP-2 and MMP-9 were lower in siPKCζ/ MDA-MB-231 group than those in scr/ MDA-MB-231 group, and the in vitro invasion ability was significantly decreased (P<0.05). Conclusions PKCζ can promote the invasion and metastasis of breast canc-er, and correlated with the expression of MMP-2, MMP-9(P<0.05).