南京大学学报（自然科学版）
南京大學學報（自然科學版）
남경대학학보（자연과학판）
JOURNAL OF NANJING UNIVERSITY(NATURAL SCIENCES)
2014年
4期
425-430
,共6页
胡正雪%刘林%郭红岩%尹颖
鬍正雪%劉林%郭紅巖%尹穎
호정설%류림%곽홍암%윤영
纳米ZnO%微米ZnO%活性氧%脂质过氧化物%抗氧化系统
納米ZnO%微米ZnO%活性氧%脂質過氧化物%抗氧化繫統
납미ZnO%미미ZnO%활성양%지질과양화물%항양화계통
Nano ZnO%Micro ZnO%reactive oxygen species%lipid peroxides%antioxidant system
以鲫鱼Carassius auratus 为实验生物,采用电子顺磁共振等多种检测手段,比较研究了纳米和微米ZnO腹腔注射14 d后,其在鲫鱼肝脏和脑部的分布情况以及毒性效应.研究发现,相同浓度的纳米和微米 ZnO 作用下,纳米ZnO处理组肝脏和脑部Zn含量高于微米ZnO处理组；纳米ZnO对肝脏羟基自由基,肝脏和脑部 MDA及 GSH 的诱导率高于微米ZnO；纳米ZnO对肝脏和脑部SOD活性的抑制作用也高于微米ZnO.但不同浓度纳米ZnO对羟基自由基和MDA的诱导效应及对SOD活性的抑制作用并未随浓度的升高而线性增加.结果表明：相比于微米 ZnO,纳米ZnO更易进入肝脏和脑部,进而产生更强的毒性效应.但纳米ZnO的毒性并没有随浓度升高而线性增加.
以鯽魚Carassius auratus 為實驗生物,採用電子順磁共振等多種檢測手段,比較研究瞭納米和微米ZnO腹腔註射14 d後,其在鯽魚肝髒和腦部的分佈情況以及毒性效應.研究髮現,相同濃度的納米和微米 ZnO 作用下,納米ZnO處理組肝髒和腦部Zn含量高于微米ZnO處理組；納米ZnO對肝髒羥基自由基,肝髒和腦部 MDA及 GSH 的誘導率高于微米ZnO；納米ZnO對肝髒和腦部SOD活性的抑製作用也高于微米ZnO.但不同濃度納米ZnO對羥基自由基和MDA的誘導效應及對SOD活性的抑製作用併未隨濃度的升高而線性增加.結果錶明：相比于微米 ZnO,納米ZnO更易進入肝髒和腦部,進而產生更彊的毒性效應.但納米ZnO的毒性併沒有隨濃度升高而線性增加.
이즉어Carassius auratus 위실험생물,채용전자순자공진등다충검측수단,비교연구료납미화미미ZnO복강주사14 d후,기재즉어간장화뇌부적분포정황이급독성효응.연구발현,상동농도적납미화미미 ZnO 작용하,납미ZnO처리조간장화뇌부Zn함량고우미미ZnO처리조；납미ZnO대간장간기자유기,간장화뇌부 MDA급 GSH 적유도솔고우미미ZnO；납미ZnO대간장화뇌부SOD활성적억제작용야고우미미ZnO.단불동농도납미ZnO대간기자유기화MDA적유도효응급대SOD활성적억제작용병미수농도적승고이선성증가.결과표명：상비우미미 ZnO,납미ZnO경역진입간장화뇌부,진이산생경강적독성효응.단납미ZnO적독성병몰유수농도승고이선성증가.
Nano ZnO has attracted increasing concerns because of its widespread use and unique toxic potential.While,so far most researches focused on the concentration effects but not the size effects of Nano ZnO.The purpose of this study was to compare the toxic effects of Nano ZnO and Micro ZnO to Carassiusauratus.Fourteen days after the intraperitoneal injection of Nano ZnO(1,12.5,50 mg ZnO/kg)and Micro ZnO(12.5 mg ZnO/kg),the distribution and toxic effects of Nano and Micro ZnO on Carassiusauratus liver and brain were studied.The particle size of both Nano and Micro ZnO was determined by transmission electron microscopy (TEM).And the free radicals (·OH )generation was determined by electron paramagnetic resonance (EPR).Several antioxidant biomarkers such as superoxide dysmutase (SOD),glutathione S-transferases(GST),glutathione(GSH)and malondialdehyde(MDA)were measured,and the effects of Nano and Micro ZnO on Zn content in liver and brain of C.auratus were also detected.We found that both Nano and Micro ZnO could induce the production of·OH.As compared to Micro ZnO,exposure to 12.5 mg ZnO/kg Nano ZnO caused more accumulation of ·OH in the liver of C.auratus.Accordingly,Nano ZnO induced the synthesis of MDA and suppressed the SOD activities in the liver and brain of C.auratus.Further,Nano ZnO effects on GSH were much higher than those of Micro ZnO,suggesting more severe oxidative stress caused by Nano ZnO under the same concentration.The contents of Zn in both liver and brain of C.auratus exposed to Nano ZnO were also much higher than those exposed to Micro ZnO.However,there was no linear relationship between the increased concentrations of Nano ZnO and the induction of ·OH and MDA and the inhibition of SOD activities.Overall,the results indicated that Nano ZnO could be more effectively taken into the brain and liver of Carassius auratus than Micro ZnO,resulting in more toxic effects,but the toxic effects of Nano ZnO didn’t have a linear relationship with the concentrations.
