CAJ | 학술논문

畜禽粪便和生活污水是造成水体污染的主要因素之一。快速、准确地确定污染源对于水体污染防治尤为重要。近年来，基于线粒体DNA(mtDNA)种属特异性的污染物溯源技术已成为国际研究热点。本文综述了水体粪便污染的 mtDNA溯源技术的理论基础及其应用进展，并通过与微生物溯源技术进行比较，分析了 mtDNA作为溯源标记物的优势以及存在的问题，从而为实际环境水体中粪便污染的生物溯源工作提供参考。
축금분편화생활오수시조성수체오염적주요인소지일。쾌속、준학지학정오염원대우수체오염방치우위중요。근년래，기우선립체DNA(mtDNA)충속특이성적오염물소원기술이성위국제연구열점。본문종술료수체분편오염적 mtDNA소원기술적이론기출급기응용진전，병통과여미생물소원기술진행비교，분석료 mtDNA작위소원표기물적우세이급존재적문제，종이위실제배경수체중분편오염적생물소원공작제공삼고。
Water environment is often contaminated by human and animal feces containing nutrients including carbon, nitrogen and phosphorus,as well as virulent pathogens.Correct and rapid identification of the pollution sources is considered crucial to mitigate the influence of such contamination.Mitochondrial DNA,coming directly from a host cell,is considered as an excellent genetic marker for fecal source tracking(FST)due to its high host-specificity and detection sensitivity.This paper summarizes the advances and applications of mitochondrial DNA source tracking based on comparisons with microbial source tracking,highlighting the potentials and limitations of mitochondrial DNA source tracking.As a new method,mitochondrial DNA source tracking has to be improved based on more thorough researches before it is used for routine water analysis in the future.