CAJ | 학술논문

目的：研究微弧氧化纯钛表面对MC3 T3-E 1细胞胶原分泌和成骨相关基因表达的影响。方法：纯钛圆片分为2个组：微弧氧化组（MAO）和抛光组（PT），培养板表面（TC）作为对照组。用场发射扫描电镜（FESEM）观察试样表面形态，表面粗糙度仪测量其表面粗糙度。将细胞接种于3组材料表面体外培养，培养第12天用粘胶纤维红染色检测细胞胶原分泌，第16天RT-PCR检测细胞成骨相关基因表达。结果：纯钛表面经微弧氧化处理后形成一层多孔氧化层，表面粗糙度增加（P＜0．01）。细胞在MAO组试件表面的胶原分泌高于PT或TC组表面（P＜0．05）。细胞在MAO组试件表面OSX、COL-Iα1及OPN基因的表达均稍高于PT表面。结论：相比于纯钛抛光表面，微弧氧化纯钛表面更能促进MC3 T3-E 1细胞胶原分泌。
목적：연구미호양화순태표면대MC3 T3-E 1세포효원분비화성골상관기인표체적영향。방법：순태원편분위2개조：미호양화조（MAO）화포광조（PT），배양판표면（TC）작위대조조。용장발사소묘전경（FESEM）관찰시양표면형태，표면조조도의측량기표면조조도。장세포접충우3조재료표면체외배양，배양제12천용점효섬유홍염색검측세포효원분비，제16천RT-PCR검측세포성골상관기인표체。결과：순태표면경미호양화처리후형성일층다공양화층，표면조조도증가（P＜0．01）。세포재MAO조시건표면적효원분비고우PT혹TC조표면（P＜0．05）。세포재MAO조시건표면OSX、COL-Iα1급OPN기인적표체균초고우PT표면。결론：상비우순태포광표면，미호양화순태표면경능촉진MC3 T3-E 1세포효원분비。
Objective:To investigate the effects of titanium surface modified by micro-arc oxidation (MAO)on the collagen secre-tion and the expression of osteogenesis-related genes of MC3T3-E1 cells.Methods:Pure titanium discs were divided into two groups:Titanium with modified surface by MAO(MAO group)and titanium with polished surface(PT group).Tissue culture polysty-rene plates (TC)were used as controls.Surface morphology of the samples was examined by field-emission scanning electron micros-copy(FESEM).Surface roughness of samples was measured by surface roughness meter.MC3T3-E1 cells were cultured on the sur-face of the samples and the collagen secretion on the samples was measured by sirius red-based colorimetric microassay after 12 days of culture.On day 16 of culture,the expression of osteogenesis-related genes was examined by RT-PCR.Results:A porous oxide layer was observed on the surface of MAO treated samples and the surface roughness was more than that of PT group(P<0.01 ). Cells in MAO group showed higher collagen secretion than in PT or TC groups(P<0.05).MAO treated surface induced higher OSX, COL-Iα1 and OPN expression than PT.Conclusion:MAO treated titanium surface may induce more collagen secretion of MC3T3-E1 cells than PT.