CAJ | 학술논문

目的：观察外源性的血小板衍生生长因子-BB （rhPDGF-BB）与转化生长因子-β1（rhTGF-β1）联合应用对大鼠正畸牙压力侧破骨细胞FAK mRNA 基因水平的表达变化。方法：将160只SD大鼠随机分为实验组和对照组。每组又根据检测时间点不同分为5个小组（n＝16），分别建立正畸牙移动模型。实验组从加力第1天开始在正畸牙颊侧黏膜下隔日注射rhP-DGF-BB 10 ng与rhTGF-β15 ng，对照组注射相同容量的PBS。加力后1、4、7、10和14 d分别处死每大组的一小组大鼠。收集标本，用TRAP染色观察压力侧破骨细胞数量的变化，实时定量PCR（RT-PCR）检测FAK mRNA基因的表达。结果：rhP-DGF-BB及rhTGF-β1联合注射明显促进压力侧破骨细胞数目的增加（P＜0．05）；破骨细胞内FAK mRNA基因量的表达7 d前增加（P＜0．05），7 d后逐渐降低，14 d时与对照组无显著差异（P＞0．05）。结论：外源性PDGF-BB和TGF-β1联合应用促进正畸牙压力侧破骨细胞增殖，增加了破骨细胞内FAK mRNA基因表达。
목적：관찰외원성적혈소판연생생장인자-BB （rhPDGF-BB）여전화생장인자-β1（rhTGF-β1）연합응용대대서정기아압력측파골세포FAK mRNA 기인수평적표체변화。방법：장160지SD대서수궤분위실험조화대조조。매조우근거검측시간점불동분위5개소조（n＝16），분별건립정기아이동모형。실험조종가력제1천개시재정기아협측점막하격일주사rhP-DGF-BB 10 ng여rhTGF-β15 ng，대조조주사상동용량적PBS。가력후1、4、7、10화14 d분별처사매대조적일소조대서。수집표본，용TRAP염색관찰압력측파골세포수량적변화，실시정량PCR（RT-PCR）검측FAK mRNA기인적표체。결과：rhP-DGF-BB급rhTGF-β1연합주사명현촉진압력측파골세포수목적증가（P＜0．05）；파골세포내FAK mRNA기인량적표체7 d전증가（P＜0．05），7 d후축점강저，14 d시여대조조무현저차이（P＞0．05）。결론：외원성PDGF-BB화TGF-β1연합응용촉진정기아압력측파골세포증식，증가료파골세포내FAK mRNA기인표체。
Objective:To study the effects of recombinant human platelet-derived growth factor-BB(rhPDGF-BB)combined with recombinant human transforming growth factor-β1 (rhTGF-β1 )on the expression of FAK mRNA of osteoclasts in the alveolar bone on the pressure side of orthodontic teeth in rats.Methods:Orthodontic tooth movement model was established in 160 male SD rats.The rats in experimental group were treated by injection of 10 ng rhPDGF-BB and 5 ng rhTGF-β1 in the buccal submucosal area of the mo-lar every other day from day 1 afterburner,while those in control group received equivalent volumes of PBS.The rats were sacrificed at 1,4,7,10 and 14 days(n=16)after appliance placement.Specimens were collected.Osteoclasts in the alveolar bone on the pres-sure side of the orthodontic teeth were observed by TRAP staining,the FAK mRNA expression was quantified by quantitative RT-PCR.Results:rhPDGF-BB combined with rhTGF-β1 significantly promoted an increasing number of osteoclasts on the compressing side(P<0.05),increased the expression of FAK mRNA at day 4 and 7(P<0.05),then decresed it to the control level(P>0.05) at day 14.Conclusion:Combination of rhPDGF-BB and rhTGF-β1 can increase the number of osteoclasts in the alveolar bone on compressing side,and promote FAK mRNA expression in osteoclasts.