CAJ | 학술논문

目的：研究橙皮苷在大鼠体内的防龋作用。方法：纸片扩散法检测橙皮苷对远缘链球菌（S．sobrinus）的最小抑菌浓度（MIC），接种S．sobrinus建立大鼠龋病模型，分别用1／2 MIC橙皮苷、0．12％洗必泰、无菌蒸馏水处理大鼠口腔，菌落计数法动态监测S．sobrinus水平、Keyes龋齿评分法、激光龋齿诊断仪（DIAGNOdent）探测荧光值评价不同处理方法对大鼠龋病发生的影响。结果：橙皮苷的MIC为8 mg／ml；S．sobrinus水平：橙皮苷组与蒸馏水组相比差异无统计学意义（P＞0．05）；Keyes记分：橙皮苷组光滑面及窝沟E级龋损记分低于蒸馏水组（P＜0．05），橙皮苷组窝沟Ds级、Dm级龋损记分低于蒸馏水组（P＜0．05），高于洗必泰组（P＜0．05）；DIAGNOdent探测荧光值：橙皮苷组龋损程度低于蒸馏水组（P＜0．05），高于洗必泰组（P＞0．05）。结论：1／2 MIC橙皮苷在不影响大鼠口内菌群水平的前提下，可减少大鼠磨牙龋病形成及减轻龋损程度。
목적：연구등피감재대서체내적방우작용。방법：지편확산법검측등피감대원연련구균（S．sobrinus）적최소억균농도（MIC），접충S．sobrinus건립대서우병모형，분별용1／2 MIC등피감、0．12％세필태、무균증류수처리대서구강，균락계수법동태감측S．sobrinus수평、Keyes우치평분법、격광우치진단의（DIAGNOdent）탐측형광치평개불동처리방법대대서우병발생적영향。결과：등피감적MIC위8 mg／ml；S．sobrinus수평：등피감조여증류수조상비차이무통계학의의（P＞0．05）；Keyes기분：등피감조광활면급와구E급우손기분저우증류수조（P＜0．05），등피감조와구Ds급、Dm급우손기분저우증류수조（P＜0．05），고우세필태조（P＜0．05）；DIAGNOdent탐측형광치：등피감조우손정도저우증류수조（P＜0．05），고우세필태조（P＞0．05）。결론：1／2 MIC등피감재불영향대서구내균군수평적전제하，가감소대서마아우병형성급감경우손정도。
Objective:To investigate the anticaries effects of hesperidin in rats.Methods:The MIC of hesperidin against Streptococ-cus sobrinus(S.sobrinus)was explored with disc diffusion method.The rats with artificial caries were administered with 1/2 MIC hes-peridin,0.12%Chlorhexidine and distilled water respectively.The results of S.sobrinus level,Keyes scoring and DIAGNOdent exam-ination were used to evaluate the effects of hesperidin on S.sobrinus and caries development.Results:The MIC of hesperidin agaist S. sobrinus was 8 mg/ml.The S.sobrinus level was not statistically different between hesperidin group and negative control group(P>0. 05).Keyes scores of grade E of smooth and pit and fissure caries in hesperidin group were lower than in negative control(P<0.05), those of grade Ds and Dm of pit and fissure caries in hesperidin group were lower than in negative control(P<0.05 ),while higher than in chlorhexidine group(P<0.05).Hesperidin showed significant anti-caries effect(P<0.05)examined by DIAGNOdent.Con-clusion:Hesperidin at 1/2 MIC has anti-caries effect in rats without influence of the oral microecological balance.