CAJ | 학술논문

目的构建左旋多巴-聚酰胺-胺(PAMAM)树突状大分子共轭体，评价其在α-突触核蛋白(α-Syn)纤丝化过程中的作用。方法采用左旋多巴对PAMAM树突状大分子进行表面修饰，合成端基改性产物左旋多巴- PAMAM树突状大分子共轭体。应用超视光谱和衰减傅里叶变换红外光谱仪(FTIR)鉴定左旋多巴-PAMAM树突状大分子共轭体合成，硫磺素含量测定及FTIR评价左旋多巴- PAMAM树突状大分子共轭体在α-Syn纤丝化中的作用。结果超视光谱和FTIR可以鉴定共轭体是否形成，相对于单独的左旋多巴及PAMAM树突状大分子，共轭体的FTIR峰值区域吸收增加。α-Syn与PAMAM树突状大分子共孵育后，与空白对照组(硫磺素+缓冲液)比较，其硫磺素荧光染色显著减少(P<0.05)；与左旋多巴-PAMAM树突状大分子共轭体共孵育后，硫磺素荧光染色进一步减弱。α-Syn的FTIR显示，左旋多巴-PAMAM树突状大分子共轭体组随时间延长，峰值区域明显下降，且吸收率的差异发生在1636 cm-1和1655 cm-1。结论左旋多巴与PAMAM树突状大分子可以形成共轭体，左旋多巴-PAMAM树突状大分子共轭体显著抑制α-Syn纤丝化，且各时间点的效果均较单独应用左旋多巴或PAMAM树突状大分子更好。
목적구건좌선다파-취선알-알(PAMAM)수돌상대분자공액체，평개기재α-돌촉핵단백(α-Syn)섬사화과정중적작용。방법채용좌선다파대PAMAM수돌상대분자진행표면수식，합성단기개성산물좌선다파- PAMAM수돌상대분자공액체。응용초시광보화쇠감부리협변환홍외광보의(FTIR)감정좌선다파-PAMAM수돌상대분자공액체합성，류광소함량측정급FTIR평개좌선다파- PAMAM수돌상대분자공액체재α-Syn섬사화중적작용。결과초시광보화FTIR가이감정공액체시부형성，상대우단독적좌선다파급PAMAM수돌상대분자，공액체적FTIR봉치구역흡수증가。α-Syn여PAMAM수돌상대분자공부육후，여공백대조조(류광소+완충액)비교，기류광소형광염색현저감소(P<0.05)；여좌선다파-PAMAM수돌상대분자공액체공부육후，류광소형광염색진일보감약。α-Syn적FTIR현시，좌선다파-PAMAM수돌상대분자공액체조수시간연장，봉치구역명현하강，차흡수솔적차이발생재1636 cm-1화1655 cm-1。결론좌선다파여PAMAM수돌상대분자가이형성공액체，좌선다파-PAMAM수돌상대분자공액체현저억제α-Syn섬사화，차각시간점적효과균교단독응용좌선다파혹PAMAM수돌상대분자경호。
Aim To prepare and characterizeL-dopa-dendrimer conjugate, and evaluate its effect onα-synuclein ifbrillation of Parkinson’s disease.MethodsL-dopa was used to modify G4 poly amido-amine dendrimer (G4 PAMAM), and the modiifed product levodopa-dendrimer conjugate was synthesized. Fourier Transform Infrared Spectroscopy (FTIR) was used to confirm the synthesization ofL-dopa-dendrimer conjugate. The effect ofL-dopa-dendrimer conjugate on α-synuclein fibrillation was evaluated by Ultra-visible Spectroscopy and thioflavint assay andFTIR.Results There is also an increase in peak area observed in the region of FTIR inL-dopa-dendrimer conjugate compared to dendrimer orL-dopa alone.α-synuclein incubated with different drugs showed similar data that a signiifcant reduction in thiolfavint lfuorescence compared to the lfuorescence observed in the absence of the drugs, and withL-dopa-dendrimer conjugate was able to inhibit the AS fibrillation better. FTIR of α-synuclein withL-dopa -dendrimer conjugate showed that the peak area declined dramatically with respect to increase in time, and the differences in the area occurred in the absorbance around 1 636 cm-1 and 1 655 cm-1.ConclsionTheL-dopa and dendrimer might formL-dopa-dendrimer conjugate. The conjugate can be characterized by using Ultra-visible Spectroscopy and FTIR. Using thiolfavint assay and FTIR were able to measure the effect of the conjugate on α-synuclein fibrillation. And the data showed thatL-dopa-dendrimer conjugate significantly inhibitedα-synuclein ifbrillation (P<0.05) greater than dendrimer orL-dopa alone all the time.