CAJ | 학술논문

目的：通过检测14-3-3和Bcl-2蛋白在人脑胶质瘤中的表达情况,探讨其在胶质瘤发生、发展中的作用机制.方法采用免疫组化S-P法对5例正常脑组织标本和68例人脑胶质瘤标本进行14-3-3和Bcl-2蛋白检测.结果与正常脑组织比较,14-3-3蛋白在胶质瘤表达异常增强(P<0.05)；在不同病理分级中胶质瘤组织表达无统计学意义(P>0.05),随着胶质瘤恶性程度的增加,14-3-3蛋白表达的强度和范围增加(P<0.05). Bcl-2蛋白在不同级别的恶性胶质瘤中阳性表达率有差别(P<0.05),且表达强度随着胶质瘤病理分级增高呈明显增高趋势.结论14-3-3和Bcl-2蛋白对胶质瘤的恶性程度、肿瘤的病理分级有影响,可作为指导临床病理诊断及分级的参考指标.
목적：통과검측14-3-3화Bcl-2단백재인뇌효질류중적표체정황,탐토기재효질류발생、발전중적작용궤제.방법채용면역조화S-P법대5례정상뇌조직표본화68례인뇌효질류표본진행14-3-3화Bcl-2단백검측.결과여정상뇌조직비교,14-3-3단백재효질류표체이상증강(P<0.05)；재불동병리분급중효질류조직표체무통계학의의(P>0.05),수착효질류악성정도적증가,14-3-3단백표체적강도화범위증가(P<0.05). Bcl-2단백재불동급별적악성효질류중양성표체솔유차별(P<0.05),차표체강도수착효질류병리분급증고정명현증고추세.결론14-3-3화Bcl-2단백대효질류적악성정도、종류적병리분급유영향,가작위지도림상병리진단급분급적삼고지표.
Objective To explore biological significance in the occurrence and development of glioma through investigating the expression of 14-3-3 and Bcl-2 proteins in human glioma. Methods The immunohistochemical S-P method was used to detect 14-3-3 and Bcl-2 proteins in 5 normal brain examples and 68 human glioma specimens. Results Compared with that in normal brain tissue,the expression of 14-3-3 protein was upregulated obviously ( P<0. 05 ) in glioma tissues, but in different pathological grading glioma, there had no obvious difference ( P>0 . 05 ) , and the strength and scope of the 14-3-3 protein expression in glioma tissues increased with the increase in the malignant degree ( P<0 . 05 ) . The positive expression rate of Bcl-2 showed obvious differences in malignant glioma with different pathological grades (P<0. 05),and the intensity of Bcl-2 expression presented a significant elevating trend with the increase in the pathological grade. Conclusion 14-3-3 and Bcl-2 proteins in human glioma tissues can affect the malignant degree and pathological grade of glioma,so that they could be used as a guide for clinical pathological diagnosis and grading.