北华大学学报（自然科学版）
北華大學學報（自然科學版）
북화대학학보（자연과학판）
JOURNAL OF BEIHUA UNIVERSITY(NATURAL SCIENCE)
2014年
5期
606-609
,共4页
Foxp3%黑色素瘤%基因转染%细胞增殖
Foxp3%黑色素瘤%基因轉染%細胞增殖
Foxp3%흑색소류%기인전염%세포증식
Foxp3%melanoma%gene transfection%cell proliferation
目的：通过检测Foxp3在黑色素瘤B16细胞中的表达及其对肿瘤细胞增殖的影响,探讨Foxp3在黑色素瘤细胞中表达的可能作用.方法采用RT-PCR和免疫组化法检测B16细胞中Foxp3在mRNA和蛋白水平的表达；采用VigoFect转染试剂将pcDNA3.1-Foxp3真核表达载体瞬时转染B16细胞,RT-PCR和流式细胞术方法分别检测转染前后Foxp3基因和蛋白的表达；MTT方法检测Foxp3高表达后对肿瘤细胞增殖的影响.结果 B16细胞在mRNA 和蛋白水平均表达Foxp3；瞬时转染后Foxp3转染组中Foxp3的表达显著高于空载体组和B16组( P<0.01)；Foxp3转染组细胞A490 nm 值在48 h和72 h与空载体组和B16组相比显著降低,差异具有统计学意义( P<0.05).结论黑色素瘤B16细胞Foxp3的表达可抑制肿瘤细胞的增殖.
目的：通過檢測Foxp3在黑色素瘤B16細胞中的錶達及其對腫瘤細胞增殖的影響,探討Foxp3在黑色素瘤細胞中錶達的可能作用.方法採用RT-PCR和免疫組化法檢測B16細胞中Foxp3在mRNA和蛋白水平的錶達；採用VigoFect轉染試劑將pcDNA3.1-Foxp3真覈錶達載體瞬時轉染B16細胞,RT-PCR和流式細胞術方法分彆檢測轉染前後Foxp3基因和蛋白的錶達；MTT方法檢測Foxp3高錶達後對腫瘤細胞增殖的影響.結果 B16細胞在mRNA 和蛋白水平均錶達Foxp3；瞬時轉染後Foxp3轉染組中Foxp3的錶達顯著高于空載體組和B16組( P<0.01)；Foxp3轉染組細胞A490 nm 值在48 h和72 h與空載體組和B16組相比顯著降低,差異具有統計學意義( P<0.05).結論黑色素瘤B16細胞Foxp3的錶達可抑製腫瘤細胞的增殖.
목적：통과검측Foxp3재흑색소류B16세포중적표체급기대종류세포증식적영향,탐토Foxp3재흑색소류세포중표체적가능작용.방법채용RT-PCR화면역조화법검측B16세포중Foxp3재mRNA화단백수평적표체；채용VigoFect전염시제장pcDNA3.1-Foxp3진핵표체재체순시전염B16세포,RT-PCR화류식세포술방법분별검측전염전후Foxp3기인화단백적표체；MTT방법검측Foxp3고표체후대종류세포증식적영향.결과 B16세포재mRNA 화단백수평균표체Foxp3；순시전염후Foxp3전염조중Foxp3적표체현저고우공재체조화B16조( P<0.01)；Foxp3전염조세포A490 nm 치재48 h화72 h여공재체조화B16조상비현저강저,차이구유통계학의의( P<0.05).결론흑색소류B16세포Foxp3적표체가억제종류세포적증식.
Objective To explore effects of Foxp3 expression on the proliferation in melanoma B16 cells by detecting the expression of Foxp3 in melanoma B16 cells and its effects on the cell proliferation. Method Foxp3 mRNA was detected by RT-PCR and Foxp3 protein was detected by immunocytochemical staining. The plasmid pcDNA3. 1-Foxp3 was transfected into B16 cells by VigoFect. Expressions of Foxp3 gene and protein before and after the transfection in B16 cells were detected by RT-PCR and flow cytometry,respectively. The effect of over-expressed Foxp3 on the proliferation of B16 cells was detected by MTT. Results Foxp3 mRNA and protein could be detected in B16 cells. The expression of Foxp3 was significantly higher in Foxp3 transferred cells than that of empty plasmid transferred cells and B16 cells(P<0. 01). The transferred cells A490 in Foxp3 group were much lower than those in empty plasmid group and B16 group at 48 h and 72 h. (P<0. 05). Conclusion The expression of Foxp3 could inhibit the cell proliferation in B16 cells.
