CAJ | 학술논문

目的：研究磁性Fe3 O4纳米颗粒在家兔体内组织超微结构的分布、血药浓度-时间曲线及对血液生化指标的影响。方法家兔耳缘静脉注射磁性Fe3 O4纳米颗粒，于不同的时间点耳缘静脉采血，测定血药浓度，计算药代动力学参数。另将24只家兔按体质量随机分成对照组、低剂量组、中剂量组、高剂量组，耳缘静脉注射不同剂量磁性Fe3 O4纳米颗粒24 h后取脏器组织及血液，测量磁性Fe3 O4纳米颗粒在家兔体内各脏器的分布，电镜观察肝、脾超微结构改变及纳米颗粒分布，并对家兔血清生化指标变化进行测定。结果纳米Fe3 O4在家兔的体内随着时间的增加其含量逐渐下降，符合药代动力学二室曲线代谢模型；纳米Fe3 O4在家兔体内分布具有特异性，主要分布在脾脏和肝脏中的吞噬细胞内，且在同一器官不同剂量组间分布也有差异；血清生化指标测定表明，家兔血清中碱性磷酸酶(ALP)、门冬氨酸氨基转移酶(AST)、尿酸(URIC)含量有所改变。结论磁性Fe3O4纳米颗粒在家兔体内主要分布在肝脏和脾脏，在组织超微结构下主要分布在吞噬细胞内，血液生化指标没有大的改变。
목적：연구자성Fe3 O4납미과립재가토체내조직초미결구적분포、혈약농도-시간곡선급대혈액생화지표적영향。방법가토이연정맥주사자성Fe3 O4납미과립，우불동적시간점이연정맥채혈，측정혈약농도，계산약대동역학삼수。령장24지가토안체질량수궤분성대조조、저제량조、중제량조、고제량조，이연정맥주사불동제량자성Fe3 O4납미과립24 h후취장기조직급혈액，측량자성Fe3 O4납미과립재가토체내각장기적분포，전경관찰간、비초미결구개변급납미과립분포，병대가토혈청생화지표변화진행측정。결과납미Fe3 O4재가토적체내수착시간적증가기함량축점하강，부합약대동역학이실곡선대사모형；납미Fe3 O4재가토체내분포구유특이성，주요분포재비장화간장중적탄서세포내，차재동일기관불동제량조간분포야유차이；혈청생화지표측정표명，가토혈청중감성린산매(ALP)、문동안산안기전이매(AST)、뇨산(URIC)함량유소개변。결론자성Fe3O4납미과립재가토체내주요분포재간장화비장，재조직초미결구하주요분포재탄서세포내，혈액생화지표몰유대적개변。
Objective To study ultramicrostructure tissue distribution, concentration-time curve and effects on serum biochemical parameters of magnetic nano-Fe3 O4 in rabbits. Methods Magnetic nano-Fe3 O4 was injected through ear vein of rabbit. The blood was got at different times after the intravenous injection. The concentration of Nano-Fe3 O4 in rabbit blood was tested by ICP-OES and the pharmacokinetic parameters were calculated. Another 24 rabbits were divided into four groups randomly according to body weight,normal control group,low-dose group, middle-dose group, high-dose group. Magnetic nano-Fe3 O4 of different doses was injected through ear vein. The blood and different organs were got at 24 hours after the intravenous injection. The distribution of Nano-Fe3 O4 in different organs were tested and ultramicrostructure liver and spleen tissues were observed by TEM, biochemical parameters of serum in rabbits were tested. Results The concentration of nano-Fe3 O4 in rabbit blood was decreased with the time,which was consistent with two compartment model of pharmacokinetic. The distribution of nano-Fe3 O4 in different organs showed its own characteristics. It was mainly distributed in spleen and liver,and its distribution was different according to different doses in the same organ. The measurement of serum biochemical parameter contents showed some changes in ALP,AST and URIC of blood. Conclusion Nano-Fe3O4 is mainly distributed in spleen and liver of rabbits and mainly concentrated in macrophage in ultramicrostructure. There is no too much change in biochemical parameters of serum.