CAJ | 학술논문

建立了高效液相色谱荧光法检测鸡组织中阿莫西林（ AMO ）的残留量。鸡组织样品经磷酸二氢钠溶液提取，乙腈去蛋白，正己烷去脂肪，饱和二氯甲烷萃取，上清液经水杨醛在酸性条件下沸水浴衍生化。以0．01 mol／L 的磷酸二氢钾溶液和乙腈溶液为流动相，梯度洗脱，流速为1 mL／min，高效液相色谱荧光检测，激发波长为358 nm，发射波长为440 nm。阿莫西林在25～1000μg／kg质量浓度范围内，线性关系良好。当阿莫西林添加水平为13～125μg／kg时，鸡组织中AMO的平均回收率在72．82％～88．82％，相对标准偏差在6．21％～9．63％；检测限、定量限分别为5μg／kg（S／N≥3）、13μg／kg（S／N≥10）。该方法操作简便，可适用于鸡组织中阿莫西林的提取和检测，且准确度和精密度均符合残留分析的要求。
건립료고효액상색보형광법검측계조직중아막서림（ AMO ）적잔류량。계조직양품경린산이경납용액제취，을정거단백，정기완거지방，포화이록갑완췌취，상청액경수양철재산성조건하비수욕연생화。이0．01 mol／L 적린산이경갑용액화을정용액위류동상，제도세탈，류속위1 mL／min，고효액상색보형광검측，격발파장위358 nm，발사파장위440 nm。아막서림재25～1000μg／kg질량농도범위내，선성관계량호。당아막서림첨가수평위13～125μg／kg시，계조직중AMO적평균회수솔재72．82％～88．82％，상대표준편차재6．21％～9．63％；검측한、정량한분별위5μg／kg（S／N≥3）、13μg／kg（S／N≥10）。해방법조작간편，가괄용우계조직중아막서림적제취화검측，차준학도화정밀도균부합잔류분석적요구。
To develop a high performance liquid chromatography with fluorescence detection ( HPLC-FLD ) method for determination of residues of amoxicillin ( AMO) in chicken tissues, AMO were extracted from chicken tissues with sodium dihydrogen phosphate solution. After being deproteinizated with acetonitrile and degreased in n-hexane saturated, the analyte was extracted by saturated dichloromethane. The supernatant was reacted with salicylaldehyde under acidic and boiling conditions. The mobile phase were 0. 01 mol/L potassium dihydrogen phosphate and acetonitrile, the flow rate was 1 mL/min. Fluorescence detection was carried out at excitation and emission wavelengths of 358 nm and 440 nm. The calibration curves were linear in the range from 25 to 1000 μg/kg. At spiked level of 13~125 μg/kg for AMO, the average recoveries was 72. 82%~88. 82% with coefficients of variation between 6.21% and 9.63%. Limit of detection ( LOD) and limit of quantitation ( LOQ) were 5 μg/kg and 13 μg/kg, respectively. This HPLC-FLD method is simple, rapid, sensitive and reliable for the determination of residues of amoxicillin in chicken tissues.