CAJ | 학술논문

为有效控制鸡大肠杆菌病，对不同省区鸡源大肠杆菌致病菌株的分布情况及耐药特征进行了调查分析，分别采用PCR法和MIC法进行了系统进化群分群检测和药敏试验，采用皮下注射进行了动物致病性试验，并采用SPSS17．0软件进行差异显著性分析。结果显示：低致病群B1（38．13％）和非致病群A（34．02％）菌株分布相对较多，其次是高致病群D（21．23％）和B2（6．16％）；鸡源大肠杆菌耐药严重，12种药物的耐药率在50％以上，多重耐药问题突出，多重耐药率高达98．86％；不同省区鸡源大肠杆菌菌株的耐药程度存在差异，个别药物（如奥格门丁、头孢噻呋等）差异显著；高致病群B2和D群菌株的耐药程度较B1和A群菌株严重，其中氧氟沙星、庆大霉素、大观霉素、头孢噻呋、磺胺异噁唑、复方新诺明、奥格门丁等7种药物差异显著（ P＜0．05）。试验表明，我国鸡源大肠杆菌高致病菌株检出率高，且耐药程度相对严重，在适宜条件下更易导致鸡群发病且较难控制。
위유효공제계대장간균병，대불동성구계원대장간균치병균주적분포정황급내약특정진행료조사분석，분별채용PCR법화MIC법진행료계통진화군분군검측화약민시험，채용피하주사진행료동물치병성시험，병채용SPSS17．0연건진행차이현저성분석。결과현시：저치병군B1（38．13％）화비치병군A（34．02％）균주분포상대교다，기차시고치병군D（21．23％）화B2（6．16％）；계원대장간균내약엄중，12충약물적내약솔재50％이상，다중내약문제돌출，다중내약솔고체98．86％；불동성구계원대장간균균주적내약정도존재차이，개별약물（여오격문정、두포새부등）차이현저；고치병군B2화D군균주적내약정도교B1화A군균주엄중，기중양불사성、경대매소、대관매소、두포새부、광알이오서、복방신낙명、오격문정등7충약물차이현저（ P＜0．05）。시험표명，아국계원대장간균고치병균주검출솔고，차내약정도상대엄중，재괄의조건하경역도치계군발병차교난공제。
In order to prevent and control chicken colibacillosis effectively, the distribution and drug-resistance of pathogenic poultry originated E.coli strains from different province were investigated and analyzed. The phylogenetic groups and antimicrobial susceptibility was detected by PCR and MIC method respectively; the animal pathogenicity test was carried out by subcutaneous injection; and the significance of difference was analyzed by SPSS17. 0. Results showed that the lowly pathogenic group B1 ( 38. 13%) and the nonpathogenic group A (34.02%)distributed more than the highly pathogenic group D(21.23%) and B2(6.16%). The resistance of chicken originated E. coli was serious, the resistant rate of 12 kinds of drugs was more than 50.0%, and multiple drug resistance problems was serious, multiple resistant rate was as high as 98. 86%. The resistance of E. coli stains from different province was different, and there was significant difference between dividual drugs (e.g. amoxycillin/clavulanic acid and ceftiofur). The resistance of the strains from highly pathogenic group B2 and D was higher than the strains from group B1 and A, and there was significant difference for 7 kinds of drugs such as OFL, GM, SPT, EFT, SF, SXT, A/C ( P<0.05) . It conclude that the ratio of highly pathogenic strains from chicken originated E. coli in China is higher, and the drug-resistance of that is relatively serious, which are likely to lead to poultry disease and difficult to control under the suitable conditions.