CAJ | 학술논문

目的：研究降钙素（ CT ）对人牙周膜细胞（ PDLCs ）的增殖、胶原合成的影响及调控途径。方法：采用含CT开放阅读框序列的重组腺病毒Ad．CT转染PDLCs，并以转染空病毒Ad．Null的PDLCs作为对照，分别用流式细胞术检测细胞周期、定量PCR和Western blot 检测CT、胶原（Ⅰ、Ⅲ、Ⅳ型）、TGF-β的mRNA及蛋白的表达；Ad．CT转染PDLCs同时给予TGF-β抑制剂LY2109761干预，分别检测细胞周期及其胶原的表达水平。结果：转染Ad．CT后PDLCs中CT表达水平较对照组显著升高（P＜0．05）， G2／S／M期细胞比例显著高于对照组（P＜0．05），Ⅰ、Ⅲ、Ⅳ型胶原蛋白以及TGF-β的表达显著高于对照组（P＜0．05）。 LY2109761能显著逆转Ad．CT对于PDLCs增殖及胶原合成的促进作用。结论：CT可能通过TGF-β途径促进PDLC细胞的增殖及胶原的合成。
목적：연구강개소（ CT ）대인아주막세포（ PDLCs ）적증식、효원합성적영향급조공도경。방법：채용함CT개방열독광서렬적중조선병독Ad．CT전염PDLCs，병이전염공병독Ad．Null적PDLCs작위대조，분별용류식세포술검측세포주기、정량PCR화Western blot 검측CT、효원（Ⅰ、Ⅲ、Ⅳ형）、TGF-β적mRNA급단백적표체；Ad．CT전염PDLCs동시급여TGF-β억제제LY2109761간예，분별검측세포주기급기효원적표체수평。결과：전염Ad．CT후PDLCs중CT표체수평교대조조현저승고（P＜0．05）， G2／S／M기세포비례현저고우대조조（P＜0．05），Ⅰ、Ⅲ、Ⅳ형효원단백이급TGF-β적표체현저고우대조조（P＜0．05）。 LY2109761능현저역전Ad．CT대우PDLCs증식급효원합성적촉진작용。결론：CT가능통과TGF-β도경촉진PDLC세포적증식급효원적합성。
AIM:To investigate the effect of calcitonin ( CT) on the proliferation and collagen synthesis of periodontal ligament cells (PDLCs), and to explore the possible signal pathway involved .METHODS: CT gene re-combinant adenoviruses ( Ad.CT) was constructed and PDLCs were transfected with Ad .CT alone or co-treated with LY2109761.Cells transfected with Ad.Null served as the controls.Flow cytometry was used to observe cell cycle of the PDLCs.The mRNA and protein expression of CT, collagen (type I, III and IV) and transforming growth factor-β( TGF-β) were measured by RT-PCR and western blot .RESULTS: CT mRNA and protein expression in Ad .CT group was significantly higher than that in Ad .Null group (P<0.05).That the proportion of S/G2/M phase cells in Ad.CT group was significantly higher than that in Ad .Null group (P<0.05).Collagen synthesis and TGF-βexpres-sion were significantly higher in Ad.CT group than those in Ad.Null group (P<0.01).Ad.CT and LY2109761 co-treated PDLCs showed significantly lower percentage of S /G2/M phase cells and than Ad .CT treated cells .CONCLU-SION:CT might promote PDLCs proliferation and increase collagen expression through TGF -βpathway .