CAJ | 학술논문

建立了同时测定饲料中21种磺胺类药物和13种喹诺酮类药物的超高效液相色谱-串联质谱方法。以2％氨水乙腈和0．1 mol／L 氢氧化钠溶液提取样品中的待测物，HLB固相萃取柱净化，液相色谱-串联质谱法测定，外标法定量。药物在1～50 ng／mL浓度范围内线性关系良好，相关系数均大于0．99。方法的检出限为0．05 mg／kg，定量限为0．10 mg／kg，在不同饲料中添加浓度范围为0．1～100 mg／kg，平均回收率为60．6％～118．6％，相对标准偏差在1．9％～15．2％之间。本方法抗干扰能力强、分析速度快、灵敏度高，适用于饲料中磺胺类和喹诺酮类药物的测定。
건립료동시측정사료중21충광알류약물화13충규낙동류약물적초고효액상색보-천련질보방법。이2％안수을정화0．1 mol／L 경양화납용액제취양품중적대측물，HLB고상췌취주정화，액상색보-천련질보법측정，외표법정량。약물재1～50 ng／mL농도범위내선성관계량호，상관계수균대우0．99。방법적검출한위0．05 mg／kg，정량한위0．10 mg／kg，재불동사료중첨가농도범위위0．1～100 mg／kg，평균회수솔위60．6％～118．6％，상대표준편차재1．9％～15．2％지간。본방법항간우능력강、분석속도쾌、령민도고，괄용우사료중광알류화규낙동류약물적측정。
A method based on ultra performance liquid chromatography-tandem mass spectrometry ( UPLC-MS/MS) has been developed for the determination of sulfonamides and quinolones in feeds. The analyte was extracted with acetonitrile containing 2% ammonia and 0. 1 mol/L sodium hydroxide solution, and cleaned by HLB solid phase extraction cartridge. Quantification of the analyte was achieved by UPLC-MS/MS with multiple reaction monitoring ( MRM ) using external standard method. The calibration curves of the drugs were linear in the concentration ranges of 1 ~ 50 ng/mL with correlation coefficients more than 0. 99. Detection limits and quantification limits of the method for the analyte were 0. 05 mg/kg and 0. 1 mg/kg in feeds. The average recoveries ranged from 60.6% to 118.6% at the spiked level of 0.1~100 mg/kg in feeds. The relative standard deviation was from 1. 9% to 15. 2%. With the advantages of good anti-interference ability, rapidness and sensitivity, the method adapted to the determination of sulfonamides and quinolones in feeds.