中国兽药杂志
中國獸藥雜誌
중국수약잡지
CHINESE JOURNAL OF VETERINARY DRUG
2014年
10期
36-43
,共8页
李慧素%杜红鸽%吴宁鹏%臧和英%付盼盼
李慧素%杜紅鴿%吳寧鵬%臧和英%付盼盼
리혜소%두홍합%오저붕%장화영%부반반
解热镇痛抗炎类药物%峰纯度检查%光谱相似度%高效液相色谱-二极管阵列检测器
解熱鎮痛抗炎類藥物%峰純度檢查%光譜相似度%高效液相色譜-二極管陣列檢測器
해열진통항염류약물%봉순도검사%광보상사도%고효액상색보-이겁관진렬검측기
antipyretic- analgesic and anti-inflammatory drugs%peak purity test%spectral similarity%HPLC-P DA
建立了7种兽药中非法添加对乙酰氨基酚、安乃近、地塞米松和地塞米松磷酸钠药物的HPLC-PDA法。采用十八烷基键合硅胶为填充剂,磷酸二氢钠缓冲液(磷酸二氢钠3.0 g加水至1000 mL,加三乙胺1 mL,用氢氧化钠调pH值至7.0±0.2)-甲醇(80∶20)为流动相,梯度洗脱,二极管阵列检测器进行全扫描和240 nm波长测定,并采用峰纯度检查和光谱相似度检查辅助对照品比对方法,对四种目标分析物进行确证。结果显示,4种解热镇痛抗炎药物与其他物质峰分离良好,在测定范围内线性关系良好,平均回收率为73.5%~119.2%,RSD为0.1%~5.8%。本方法准确、可靠、重现性好,可用于兽药制剂中非法添加四种解热镇痛抗炎药物的定性和定量检测。
建立瞭7種獸藥中非法添加對乙酰氨基酚、安迺近、地塞米鬆和地塞米鬆燐痠鈉藥物的HPLC-PDA法。採用十八烷基鍵閤硅膠為填充劑,燐痠二氫鈉緩遲液(燐痠二氫鈉3.0 g加水至1000 mL,加三乙胺1 mL,用氫氧化鈉調pH值至7.0±0.2)-甲醇(80∶20)為流動相,梯度洗脫,二極管陣列檢測器進行全掃描和240 nm波長測定,併採用峰純度檢查和光譜相似度檢查輔助對照品比對方法,對四種目標分析物進行確證。結果顯示,4種解熱鎮痛抗炎藥物與其他物質峰分離良好,在測定範圍內線性關繫良好,平均迴收率為73.5%~119.2%,RSD為0.1%~5.8%。本方法準確、可靠、重現性好,可用于獸藥製劑中非法添加四種解熱鎮痛抗炎藥物的定性和定量檢測。
건립료7충수약중비법첨가대을선안기분、안내근、지새미송화지새미송린산납약물적HPLC-PDA법。채용십팔완기건합규효위전충제,린산이경납완충액(린산이경납3.0 g가수지1000 mL,가삼을알1 mL,용경양화납조pH치지7.0±0.2)-갑순(80∶20)위류동상,제도세탈,이겁관진렬검측기진행전소묘화240 nm파장측정,병채용봉순도검사화광보상사도검사보조대조품비대방법,대사충목표분석물진행학증。결과현시,4충해열진통항염약물여기타물질봉분리량호,재측정범위내선성관계량호,평균회수솔위73.5%~119.2%,RSD위0.1%~5.8%。본방법준학、가고、중현성호,가용우수약제제중비법첨가사충해열진통항염약물적정성화정량검측。
A method was established for the determination of four kinds of illegal additives antipyretic-analgesic and anti-inflammatory drugs in veterinary drug by the high performance liquid chromatography with photo-diode array detector. It was tested with C18 column, using gradient elution with sodium dihydrogen phosphate buffer-methanol(80 ∶ 20,V/V) as the mobile phase. The extract wavelength was 240 nm from the full scan, peak purity test and spectral similarity were helped to identify the four kinds of target analytes. Results showed that four kinds of target analytes were finely separated, and all the compounds showed good linearity in the concentration range of detection. The mean recovery of four antipyretics were 73.5%~119.2%,RSD were 0.1%~5.8%. In conclusion, the method was accurate and reproducible, and provided a reliable way for detecting antipyretic- analgesic and anti-inflammatory drugs adulterated in veterinary drug.