CAJ | 학술논문

为研制鸡产蛋下降综合症病毒（ EDS-76）检测用标准抗原及标准多抗血清，以EDS-76国际通用毒株AV-127株为抗原，进行了EDS-76病毒抗原纯化及多抗血清制备。研究表明，经氯仿抽提，并经PEG浓缩处理病毒的尿囊液可获得良好的纯化效果，纯化病毒背景干净，血凝效价达19Log2。以此研制出的鸡抗 EDS-76多抗血清血凝抑制效价达13Log2，间接免疫荧光效价达1∶2000，Western-blot试验分析表明该多抗血清可以识别两个分子大小在50 kD至85 kD之间的蛋白抗原，HI及IFA 试验发现所制备的特异性，多抗血清与12种常见禽病病原（ NDV、AIV-A、ALV-J、ALV、GPV、REV、MDV、IBV、CAV、IBDV、TMUV、REOV）均不反应。本研究建立的快速简便的EDS-76病毒纯化方法以及获得的抗EDS-76多抗血清，为进一步研制EDS-76标准抗原以及标准多抗血清提供了有效的方法与材料。
위연제계산단하강종합증병독（ EDS-76）검측용표준항원급표준다항혈청，이EDS-76국제통용독주AV-127주위항원，진행료EDS-76병독항원순화급다항혈청제비。연구표명，경록방추제，병경PEG농축처리병독적뇨낭액가획득량호적순화효과，순화병독배경간정，혈응효개체19Log2。이차연제출적계항 EDS-76다항혈청혈응억제효개체13Log2，간접면역형광효개체1∶2000，Western-blot시험분석표명해다항혈청가이식별량개분자대소재50 kD지85 kD지간적단백항원，HI급IFA 시험발현소제비적특이성，다항혈청여12충상견금병병원（ NDV、AIV-A、ALV-J、ALV、GPV、REV、MDV、IBV、CAV、IBDV、TMUV、REOV）균불반응。본연구건립적쾌속간편적EDS-76병독순화방법이급획득적항EDS-76다항혈청，위진일보연제EDS-76표준항원이급표준다항혈청제공료유효적방법여재료。
To prepare standard antigen and polyclonal antibodies for detection of chicken egg drop syndrome-76 virus ( EDS-76) , we developed a rapid method of virus purification with international strain AV-127. Our results showed that the purified virus particles could be obtained by chloroform extraction and PEG concentration from allantoic fluid. The HA titer of the purified virus reached to 19Log2. The polyclonal antibodies to EDS-76 generated here showed 13Log2 in HI titer and 1 ∶ 2000 in IFA titer. Western-blot analysis results showed the same pattern to the available commercial sera against EDS-76. Moreover, the generated polyclonal antibodies against EDS-76 did not show cross-reaction with 12 other avian pathogens( NDV, AIV-A, ALV-J, ALV, GPV, REV, MDV, IBV, CAV, IBDV, TMUV, REOV) . Overall, our rapid viral purification method provides efficient tools and materials in developing standard antigen and antibodies for detection of EDS-76 in future.