CAJ | 학술논문

目的：探讨线粒体融合蛋白-2(mfn2)和肿瘤转移抑制基因（nm23）在膀胱癌组织中的表达及与临床病理特征间的关系。方法选取65例膀胱癌标本，其中男50例，女15例。TNM分期：Ⅰ期47例、Ⅱ期10例、Ⅲ期5例、Ⅳ3例，另择正常膀胱组织、良性膀胱肿瘤标本各15例作为对照。应用免疫组化SP法检测mfn2和nm23的表达情况，并分析其在膀胱癌组织中的表达与临床病理特征之间的关系。结果膀胱癌组织中mfn2的阳性表达率均高于正常和良性膀胱组织，nm23的阳性表达率均低于正常和良性膀胱组织（均P＜0.05）。膀胱癌组织中mfn2的表达在不同分化程度、TNM分期间的差异有统计学意义，在不同年龄、性别、淋巴结转移情况和临床分期间的差异无统计学意义。nm23在不同TNM分期、淋巴结转移情况和临床分期间的差异有统计学意义，在不同年龄、性别和分化程度间的差异无统计学意义。结论 mfn2在膀胱癌中高表达，mfn2与膀胱癌的发生、发展密切相关；nm23在膀胱癌中低表达，可作为预测膀胱癌转移及其预后的参考指标。
목적：탐토선립체융합단백-2(mfn2)화종류전이억제기인（nm23）재방광암조직중적표체급여림상병리특정간적관계。방법선취65례방광암표본，기중남50례，녀15례。TNM분기：Ⅰ기47례、Ⅱ기10례、Ⅲ기5례、Ⅳ3례，령택정상방광조직、량성방광종류표본각15례작위대조。응용면역조화SP법검측mfn2화nm23적표체정황，병분석기재방광암조직중적표체여림상병리특정지간적관계。결과방광암조직중mfn2적양성표체솔균고우정상화량성방광조직，nm23적양성표체솔균저우정상화량성방광조직（균P＜0.05）。방광암조직중mfn2적표체재불동분화정도、TNM분기간적차이유통계학의의，재불동년령、성별、림파결전이정황화림상분기간적차이무통계학의의。nm23재불동TNM분기、림파결전이정황화림상분기간적차이유통계학의의，재불동년령、성별화분화정도간적차이무통계학의의。결론 mfn2재방광암중고표체，mfn2여방광암적발생、발전밀절상관；nm23재방광암중저표체，가작위예측방광암전이급기예후적삼고지표。
Objective To research the expression of mitofusin-2 (mfn2) and tumor metastasis suppressor genes (nm23)in bladder cancer cells and its correlation with clinical pathological feature. Methods Immunohistochemistry was used to measure the expression of mfn2 and nm23.Sixty-five cases of bladder cancer were sampled,which include fif-ty cases of male and fifteen cases of female. TNM stage:Forty-seven cases were in stage I;Ten cases were in stage II; Five cases were in stageⅢ; Three cases were in stageⅣ. Other fifteen cases were sampled from normal bladder or benign tumor of bladder as control . All cases were collected from department of pathology,affiliated Hospital of hebei union university. Results The positive expression rate of mfn2 in bladder cancer tissues was significantly higher than those in normal blad-der tissues and benign tumor of bladder(χ2=32.528,P<0.05);The positive expression rate of nm23 in bladder cancer tis-sues was significantly lower than those in normal bladder tissues and benign tumor of bladder (χ2=19.719,P<0.05);the high expression level of mfn2 in bladder cancer was associated with tumor differentiation and TNM stage(P<0.05),but not corre-lated with age,sex,lymph node metastasis and clinical grade. The low level of nm23 was associated with TNM stage,clinical grade and LN metastasis. Conclusion The positive expression rate of mfn2 was increased in bladder cancer. It indicated that there was a close relationship between mfn2 and the occurrence and development of bladder cancer;The expression rate of nm23 was decreased in bladder cancer,it may be a predictor for metastasis and prognosis of bladder cancer.