CAJ | 학술논문

目的：观察脓毒症急性肾损伤大鼠肾组织及血清巨噬细胞移动抑制因子（MIF）的表达规律，并探讨血必净注射液对脓毒症大鼠MIF表达的影响及急性肾损伤的防治作用。方法采用盲肠结扎穿孔术（CLP）制备脓毒症模型。健康SD大鼠80只按随机数字表法分为假手术组（n=16）、模型组(n=32)和血必净组（n=32），分别于术后2、8、24和48 h 4个时间点处死取材。采用实时定量PCR检测肾组织MIF mRNA表达，酶联免疫法检测血MIF表达；同时用生化分析仪测定血清肌酐（Cr）水平。结果与假手术组比较，模型组大鼠肾组织MIF mRNA表达于CLP后8、24、48 h显著升高（P<0.05），血清MIF及Cr水平于CLP后24、48 h显著增高（P<0.05）；与模型组比较，血必净组大鼠肾组织 MIF mRNA 于2、8、24、48 h 明显下降（P <0.05），血清 MIF 及 Cr 水平于24、48 h 亦显著下降（P<0.05）。结论 MIF可能作为晚期炎症因子参与脓毒症大鼠急性肾损伤的病生理过程。血必净注射液可抑制MIF的表达，对脓毒症大鼠肾损伤具有保护作用。
목적：관찰농독증급성신손상대서신조직급혈청거서세포이동억제인자（MIF）적표체규률，병탐토혈필정주사액대농독증대서MIF표체적영향급급성신손상적방치작용。방법채용맹장결찰천공술（CLP）제비농독증모형。건강SD대서80지안수궤수자표법분위가수술조（n=16）、모형조(n=32)화혈필정조（n=32），분별우술후2、8、24화48 h 4개시간점처사취재。채용실시정량PCR검측신조직MIF mRNA표체，매련면역법검측혈MIF표체；동시용생화분석의측정혈청기항（Cr）수평。결과여가수술조비교，모형조대서신조직MIF mRNA표체우CLP후8、24、48 h현저승고（P<0.05），혈청MIF급Cr수평우CLP후24、48 h현저증고（P<0.05）；여모형조비교，혈필정조대서신조직 MIF mRNA 우2、8、24、48 h 명현하강（P <0.05），혈청 MIF 급 Cr 수평우24、48 h 역현저하강（P<0.05）。결론 MIF가능작위만기염증인자삼여농독증대서급성신손상적병생리과정。혈필정주사액가억제MIF적표체，대농독증대서신손상구유보호작용。
Objective To investigate the expression of macrophage migration inhibitory factor (MIF) in serum and renal tissue of septic rats with actue kidney injury (AKI), and to explore the effect of Chinese traditional medicine-Xuebijing injection on MIF expression as well as on acute kidney injury in rats with sepsis. Methods Sepsis model was reproduced in rats with cecal ligation and puncture (CLP).Eighty healthy SD rats were randomly divided into three groups:sham operation group(n=16), CLP model group (n=32), and xuebijing group(n=32). All rats were sacrificed at either 2, or 8, or 24 and or 48 hours after operations.MIF mRNA levels in renal tissues of septic rats were semi-quantified by Real-time PCR.The content of MIF in serum was determined by enzyme linked immunosorbent assay (ELISA). Serum creatinine (Cr) contents were measured by automatic biochemistry analyze. Results Compared with sham operation group, transcription of MIF mRNA in renal tissues of model group were significantly enhanced at 8, 24 and 48 hours after operations (P<0.01). Both contents of MIF and creatinine level in serum of model group rose obviously at 24 and 48 hours after operation (P<0.01);Compared with model group, the transcription of MIF mRNA in renal tissues of xuebijing group decrease obviously at 2, 8, 24 and 48 hours (P<0.01) and both contents of MIF and creatinine in serum of xuebijing group drop remarkably at 24 and 48 hours (P<0.01). Conclusion MIF is a kind of late cytokine which might participate in the pathogenesis of AKI in rats with sepsis.Xuebijing injection can inhibit MIF expression, and possess the protective effects on the kidney in rats with sep-sis.