CAJ | 학술논문

目的：探讨支气管冲洗液细胞块HE切片及免疫组化染色在肺癌病理诊断与鉴别诊断中的应用价值。方法：用TCT检测筛选129例支气管冲洗液阳性（包括分型明确和不明确）病例，然后制成细胞块，行HE切片及免疫组化SP法染色。抗体选用TTF-1、CK7、CK5/6、p63、Syn、CD56、Ki-67，分析两种方法分型诊断率的差异。结果：TCT检测分型诊断率为59.7%（77/129）。细胞块及免疫组化法分型诊断率为87.6%（113/129），其中鳞状细胞癌62例、腺癌29例、小细胞癌22例。细胞块HE切片及免疫组化法明显优于单纯TCT，两种方法诊断分型率具有显著性差异（ P<0.01）。结论：支气管冲洗液细胞块HE切片及免疫组化染色对肺癌的分型诊断具有一定的价值。
목적：탐토지기관충세액세포괴HE절편급면역조화염색재폐암병리진단여감별진단중적응용개치。방법：용TCT검측사선129례지기관충세액양성（포괄분형명학화불명학）병례，연후제성세포괴，행HE절편급면역조화SP법염색。항체선용TTF-1、CK7、CK5/6、p63、Syn、CD56、Ki-67，분석량충방법분형진단솔적차이。결과：TCT검측분형진단솔위59.7%（77/129）。세포괴급면역조화법분형진단솔위87.6%（113/129），기중린상세포암62례、선암29례、소세포암22례。세포괴HE절편급면역조화법명현우우단순TCT，량충방법진단분형솔구유현저성차이（ P<0.01）。결론：지기관충세액세포괴HE절편급면역조화염색대폐암적분형진단구유일정적개치。
Objective:To compare the defference between TCT and cell block in differential diagnosis of lung cancer. Methods:One hundred and twenty-nine positive cases( types of explicit and implicit)of bronchial washes were sifted by using TCT. Then put them into cell block. Cell morphology was observed by HE staining,and expression of TTF-1,CK7,CK5/6,p63,Syn,CD56,Ki-67 were detected by SP immunohistochemical assay. To analyze deffer-ences between the two methods of typing diagnosis rate. Results:The typing diagnosis rate of TCT was 59. 7%(77/129),the typing diagnosis rate of cell block was 87. 6%(113/129). Among these cases,62 cases were squamous cell carcinoma,29 cases were adenocarcinoma,22 cases were small cell carcinoma. The typing diagnosis rate of cell block was significantly higher than that of TCT(P<0. 01). Conclusion:Cell block has a certain typing diagnostic value for lung cancer.