CAJ | 학술논문

目的：建立荧光原位杂交方法（ fluorescent in situ hybridization，FISH）检测ROS1基因重排，评价检测试剂在临床样本中的检测情况。方法：依据ROS1断裂重排方式，设计、制备双色荧光探针。以人外周血培养细胞为检测对象评价ROS1基因重排检测探针的灵敏度和特异性，建立阴性阈值。以NSCLC患者的肿瘤组织样本为对象进行ROS1重排检测及性能评价。结果：建立的FISH方法在人外周血培养细胞检测中特异性和灵敏度均达到100%，在对192例临床样本制备的高密度组织芯片的检测中，筛查出1例ROS1 FISH阳性样本。结论：本研究制备的双色荧光探针可用于ROS1基因重排检测。通过ROS1重排检测，将有助于临床方案制定，辅助个体化治疗。
목적：건립형광원위잡교방법（ fluorescent in situ hybridization，FISH）검측ROS1기인중배，평개검측시제재림상양본중적검측정황。방법：의거ROS1단렬중배방식，설계、제비쌍색형광탐침。이인외주혈배양세포위검측대상평개ROS1기인중배검측탐침적령민도화특이성，건립음성역치。이NSCLC환자적종류조직양본위대상진행ROS1중배검측급성능평개。결과：건립적FISH방법재인외주혈배양세포검측중특이성화령민도균체도100%，재대192례림상양본제비적고밀도조직심편적검측중，사사출1례ROS1 FISH양성양본。결론：본연구제비적쌍색형광탐침가용우ROS1기인중배검측。통과ROS1중배검측，장유조우림상방안제정，보조개체화치료。
Objective:To develop an assay for ROS1 rearrangement detection based on FISH technology and to e-valuate the clinical value. Methods:According to ROS1 rearrangement manner,we designed and labeled dual-color FISH probes. Using peripheral blood culture cells to evaluate genetic testing sensitivity and specificity. Using clinical samples to evaluate detection performance. Results:Both specificity and sensitivity were 100% in peripheral blood culture cells. The established FISH method was able to detect successfully one positive ROS1 rearrangement sample. Conclusion:The established method of dual-color probe can be used for ROS1 testing in hematopoietic cells.