CAJ | 학술논문

目的：研究细胞因子诱导的杀伤细胞（ CIK）与树突状细胞（ DC）共培养后对神经母细胞瘤（ neuro-blastoma，NB）细胞株的杀伤作用。方法：取健康人和肿瘤患者外周血单个核细胞（ PBMC），加入不同的细胞因子分别诱导出DC和CIK细胞，用流式细胞术测定诱导培养前后DC和CIK细胞的表型，MTT法测定不同组CIK细胞对NB细胞株的杀伤活性。结果：流式细胞仪检测健康人PBMC培养后CD3+CD56+淋巴细胞百分比以及对NB细胞株的杀伤活性均显著高于肿瘤患者（ P<0.05）。此外，与单纯CIK细胞相比，DC-CIK细胞具有更强的杀伤NB细胞株的活性（ P<0.05）。结论：DC-CIK细胞是一种细胞毒作用高于单纯CIK细胞的免疫活性细胞。健康人和肿瘤患者的PBMC经诱导培养获得的CIK细胞有显著差别，为临床进一步提高CIK细胞的治疗效果提供了实验依据。
목적：연구세포인자유도적살상세포（ CIK）여수돌상세포（ DC）공배양후대신경모세포류（ neuro-blastoma，NB）세포주적살상작용。방법：취건강인화종류환자외주혈단개핵세포（ PBMC），가입불동적세포인자분별유도출DC화CIK세포，용류식세포술측정유도배양전후DC화CIK세포적표형，MTT법측정불동조CIK세포대NB세포주적살상활성。결과：류식세포의검측건강인PBMC배양후CD3+CD56+림파세포백분비이급대NB세포주적살상활성균현저고우종류환자（ P<0.05）。차외，여단순CIK세포상비，DC-CIK세포구유경강적살상NB세포주적활성（ P<0.05）。결론：DC-CIK세포시일충세포독작용고우단순CIK세포적면역활성세포。건강인화종류환자적PBMC경유도배양획득적CIK세포유현저차별，위림상진일보제고CIK세포적치료효과제공료실험의거。
Objective:To study the killing effects on neuroblastoma( NB)cells of cytokine-induced killer cells ( CIK)co-cultured with dendritic cells( DC). Methods:Peripheral blood mononuelear cells( PBMC)were isolated from healthy subjects and tumor patients. DC and CIK cells were induced by different cytokines. The phenotypes of CIK and DC cells before and after culture were determined by flow cytometry. The killing activity of different CIK groups against NB was determined by MTT. Results:The percentage of CD3+CD56+ cells as well as the cytotoxicity activity of CIK cells originated from PBMC of peripheral blood of healthy people were significantly higher than those of tumor patients(P<0. 05). In addition,compared with CIK cells,the DC-CIK cells presented a significantly higher killing effects on NB cells(P<0. 05). Conclusion:DC-CIK cell is a kind of immune cell which has a higher cyto-toxicity than that in CIK cell. CIK cells generated from PBMC of healthy subjects and tumor patients have significant differences,and provide further experimental basis for improving the clinical curative effect of CIK cells.