CAJ | 학술논문

目的：探讨小剂量紫杉醇对乏氧Hela细胞HIF-1α的抑制作用。方法：细胞乏氧模型采用四甲基偶氮唑蓝（ MTT）快速比色法检测细胞增殖率。细胞免疫荧光法和Western blot检测Hela细胞HIF-1α的表达。结果：紫杉醇使Hela细胞的增殖率降低，但乏氧状态下的Hela细胞增殖率明显高于正常状态下的细胞，两者相比差异有统计学意义（ P<0.05）。不同浓度紫杉醇可使乏氧状态下的Hela细胞绿色荧光减弱，且呈浓度依赖性。结论：紫杉醇对乏氧和常氧状态下Hela细胞均有抑制作用，乏氧使Hela细胞对紫杉醇有一定的抵抗作用。紫杉醇对乏氧状态下的Hela细胞HIF-1α的表达有抑制作用，呈浓度依赖性。
목적：탐토소제량자삼순대핍양Hela세포HIF-1α적억제작용。방법：세포핍양모형채용사갑기우담서람（ MTT）쾌속비색법검측세포증식솔。세포면역형광법화Western blot검측Hela세포HIF-1α적표체。결과：자삼순사Hela세포적증식솔강저，단핍양상태하적Hela세포증식솔명현고우정상상태하적세포，량자상비차이유통계학의의（ P<0.05）。불동농도자삼순가사핍양상태하적Hela세포록색형광감약，차정농도의뢰성。결론：자삼순대핍양화상양상태하Hela세포균유억제작용，핍양사Hela세포대자삼순유일정적저항작용。자삼순대핍양상태하적Hela세포HIF-1α적표체유억제작용，정농도의뢰성。
Objective:To investigate whether paclitaxel can inhabit HIF-1α expression in Hela cells under hy-poxia conditions. Methods:The hypoxia model of Hela,MTT assay was used to analyze cells proliferation rate,immu-nofluorescence and Western blot was used to observe the expression of HIF-1α in Hela cells. Results:Paclitaxel make the proliferation ratio of Hela cells down,in the state of hypoxia,the proliferation ratio of Hela cells was signifi-cantly higher than normoxia,there was statistically significant difference between two groups(P<0. 05). Different concentration of paclitaxel can make the green fluorescence reduced,and it has dose-dependent effect. Conclusion:Paclitaxel can inhabit the proliferation of Hela cells under normoxia and hypoxia conditions. Hypoxia Hela cells have a certain resistance to paclitaxel. Paclitaxel inhibit HIF-1αexpression in hypoxia Hela cells,and it has dose-depend-ent effect.