CAJ | 학술논문

目的：研究银杏叶提取物EGB761对U87胶质瘤细胞的促凋亡作用。方法：EGB761干预U87胶质瘤细胞后，CCK-8观察50、100和200mg/L不同浓度EGB761作用下U87细胞的增殖状况，流式细胞仪分析用AnnexinV-FITC和PI双染检测不同浓度EGB761对U87胶质瘤细胞凋亡的影响。结果：EGB761可抑制U87胶质瘤细胞的增殖，呈浓度和时间依赖性反应；EGB761100mg/L、200mg/L干预U87胶质瘤细胞后，流式细胞仪检测显示细胞凋亡率明显升高（ P<0.05）。结论：EGB761可诱导U87胶质瘤细胞凋亡。
목적：연구은행협제취물EGB761대U87효질류세포적촉조망작용。방법：EGB761간예U87효질류세포후，CCK-8관찰50、100화200mg/L불동농도EGB761작용하U87세포적증식상황，류식세포의분석용AnnexinV-FITC화PI쌍염검측불동농도EGB761대U87효질류세포조망적영향。결과：EGB761가억제U87효질류세포적증식，정농도화시간의뢰성반응；EGB761100mg/L、200mg/L간예U87효질류세포후，류식세포의검측현시세포조망솔명현승고（ P<0.05）。결론：EGB761가유도U87효질류세포조망。
Objective:To explore the contribution of EGB761 to the proliferation and apoptosis of brain glioma U87 cell in vitro. Methods:CCK-8 assay was used to measure 50,100 and 200 mg/L which its effects on the prolif-eration of U87 cells cultured with different concentrations of allicin for 12h,24h,48h,AnnexinV-FITC and PI assay by flow cytometer(FCM)were applied to detect the apoptosis induced by EGB761. The changes of cell cycle were de-tected by FCM. Results:EGB761 suppressed the proliferation of U87 glioma cells in a concentration-and time-de-pendent manner EGB761 induced cellular apoptosis of the U87 cells in a concentration-dependent manner. With EGB761 100,200mg/L intervention U87 glioma cell,flow cytometer(FCM)test showed that the cell apoptosis rate in-creased significant(P<0. 05). Conclusion:EGB761 significantly suppresses the growth of glioma cells,induces the apoptosis of U87 glioma cells. Therefore resveratrol may be considered as a possible treatment medicine for gliomas.