CAJ | 학술논문

为揭示养分添加对草原土壤微生物群落功能多样性的影响，采用 Biolog-Eco 技术，研究了在无养分添加对照（CK）和7种不同氮磷钾养分添加方式（单养分添加：N、P、K，不同组合添加：PK、NK、NP、NPK）下贝加尔针茅草原土壤微生物群落功能多样性变化。结果表明：（1）不同养分添加条件下土壤微生物群落单孔平均颜色变化率（Average Well Color Development）AWCD 值从高到低依次为 NP跃P跃NPK跃NK跃N跃CK跃K跃PK。（2）含磷添加处理（PK 除外，NP、P、NPK）和含氮添加处理（N、NK）的丰富度指数 H 均显著高于 CK、K、PK，而均匀度指数 E 和优势度指数 D 在各处理间差异不显著。（3）主成分分析结果表明，CK、K、PK、NK 处理土壤微生物群落碳源利用方式相似，而 NP、P 和 N、NPK 分别具有不同的碳源利用方式。据此可知，NP、P、NPK、N 养分添加方式可以提高土壤微生物代谢活性、物种丰富度指数和微生物群落碳源利用能力，有利于土壤微生物群落功能多样性的提高。
위게시양분첨가대초원토양미생물군락공능다양성적영향，채용 Biolog-Eco 기술，연구료재무양분첨가대조（CK）화7충불동담린갑양분첨가방식（단양분첨가：N、P、K，불동조합첨가：PK、NK、NP、NPK）하패가이침모초원토양미생물군락공능다양성변화。결과표명：（1）불동양분첨가조건하토양미생물군락단공평균안색변화솔（Average Well Color Development）AWCD 치종고도저의차위 NP약P약NPK약NK약N약CK약K약PK。（2）함린첨가처리（PK 제외，NP、P、NPK）화함담첨가처리（N、NK）적봉부도지수 H 균현저고우 CK、K、PK，이균균도지수 E 화우세도지수 D 재각처리간차이불현저。（3）주성분분석결과표명，CK、K、PK、NK 처리토양미생물군락탄원이용방식상사，이 NP、P 화 N、NPK 분별구유불동적탄원이용방식。거차가지，NP、P、NPK、N 양분첨가방식가이제고토양미생물대사활성、물충봉부도지수화미생물군락탄원이용능력，유리우토양미생물군락공능다양성적제고。
To reveal the influence of nutrient additions on functional diversity of soil microbial communities in grassland, Biolog-Eco tech-nique was used to study the changes in microbial functional diversity in Stipa Baicalensis steppe under seven different N, P and K fertilizer treatments including single and combined additions of nutrient N, P, and K.The AWCD values of the soil microbial communities followed the order:NP>P>NPK>NK>N>CK>K>PK. The richness index of microbial communities in soil was significantly higher in NP, P, NPK, NK, and N treatments than in K and PK treatments. However, evenness index(E)and dominance index(D)of the soil microbial communities were not different among the seven treatments. Principal component analysis showed that microbial carbon utilization was similar in CK, K, PK and NK treatments, but different in NP, P and N, NPK treatments. These results implied that NP, P, NPK, and N can increase the metabolic activity of microorganism, the diversities and the carbon utilization ability of soil microbial community, which improves the soil microbial functional diversity.