CAJ | 학술논문

为探讨红壤地玉米-豌豆间作种植模式对铜污染的响应机制，通过盆栽试验，研究了不同铜浓度（0、100、200、400、600 mg· kg-1）对玉米单作和玉米间作豌豆条件下植株生物量、铜含量、玉米抗氧化酶活性[超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）]及丙二醛含量（MDA）的影响。结果表明：在高 Cu2+浓度（600 mg·kg-1）胁迫下，间作玉米地上部和地下部干重较单作分别提高了20豫和36.6豫，与单作相比，随着 Cu2+浓度升高（100、200、400、600 mg·kg-1），间作模式下玉米地上部铜含量分别降低了86.81豫、44.57豫、22.01豫、11.11豫，而地下部铜含量则分别提高了78.89豫、24.79豫、35.29豫、13.31豫，且差异均达到显著水平。在不同Cu2+浓度胁迫下，玉米叶和根中的 SOD、POD、CAT 活性和 MDA 含量均随着 Cu2+浓度的增加而提高，仅 Cu2+浓度达到600 mg·kg-1时玉米体内的 CAT 活性有所下降。间作条件下玉米根的 SOD 活性较单作提高了48.07豫~117.27豫，间作玉米叶的 SOD 活性较单作提高了11.30豫~46.90豫。不同种植模式对 Cu2+胁迫条件下的玉米 POD 活性均没有显著影响。在 Cu2+（0~400 mg·kg-1）胁迫下，间作玉米叶的 CAT 活性较单作均显著提高，分别提高了71.37豫、140.40豫、229.80豫和161.75豫，间作玉米根的 CAT 活性与单作无显著差异。间作玉米根的 MDA 含量较单作降低了26.13豫~64.53豫；在100、200 mg·kg-1 Cu2+胁迫下，间作玉米叶的 MDA 含量较单作降低了0.30豫和26.24豫，但在400、600 mg·kg-1 Cu2+胁迫下，间作玉米叶的 MDA 含量较单作提高了32.62豫和93.51豫。综上所述，在一定范围的 Cu2+胁迫条件下，玉米根和叶中的抗氧化酶活性及 MDA 含量均有所提高来维持正常生长，间作模式在 Cu2+胁迫下对玉米根和叶的抗氧化酶系统能起到一定的保护作用。為探討紅壤地玉米-豌豆間作種植模式對銅汙染的響應機製，通過盆栽試驗，研究瞭不同銅濃度（0、100、200、400、600 mg· kg-1）對玉米單作和玉米間作豌豆條件下植株生物量、銅含量、玉米抗氧化酶活性[超氧化物歧化酶（SOD）、過氧化氫酶（CAT）和過氧化物酶（POD）]及丙二醛含量（MDA）的影響。結果錶明：在高 Cu2+濃度（600 mg·kg-1）脅迫下，間作玉米地上部和地下部榦重較單作分彆提高瞭20豫和36.6豫，與單作相比，隨著 Cu2+濃度升高（100、200、400、600 mg·kg-1），間作模式下玉米地上部銅含量分彆降低瞭86.81豫、44.57豫、22.01豫、11.11豫，而地下部銅含量則分彆提高瞭78.89豫、24.79豫、35.29豫、13.31豫，且差異均達到顯著水平。在不同Cu2+濃度脅迫下，玉米葉和根中的 SOD、POD、CAT 活性和 MDA 含量均隨著 Cu2+濃度的增加而提高，僅 Cu2+濃度達到600 mg·kg-1時玉米體內的 CAT 活性有所下降。間作條件下玉米根的 SOD 活性較單作提高瞭48.07豫~117.27豫，間作玉米葉的 SOD 活性較單作提高瞭11.30豫~46.90豫。不同種植模式對 Cu2+脅迫條件下的玉米 POD 活性均沒有顯著影響。在 Cu2+（0~400 mg·kg-1）脅迫下，間作玉米葉的 CAT 活性較單作均顯著提高，分彆提高瞭71.37豫、140.40豫、229.80豫和161.75豫，間作玉米根的 CAT 活性與單作無顯著差異。間作玉米根的 MDA 含量較單作降低瞭26.13豫~64.53豫；在100、200 mg·kg-1 Cu2+脅迫下，間作玉米葉的 MDA 含量較單作降低瞭0.30豫和26.24豫，但在400、600 mg·kg-1 Cu2+脅迫下，間作玉米葉的 MDA 含量較單作提高瞭32.62豫和93.51豫。綜上所述，在一定範圍的 Cu2+脅迫條件下，玉米根和葉中的抗氧化酶活性及 MDA 含量均有所提高來維持正常生長，間作模式在 Cu2+脅迫下對玉米根和葉的抗氧化酶繫統能起到一定的保護作用。
위탐토홍양지옥미-완두간작충식모식대동오염적향응궤제，통과분재시험，연구료불동동농도（0、100、200、400、600 mg· kg-1）대옥미단작화옥미간작완두조건하식주생물량、동함량、옥미항양화매활성[초양화물기화매（SOD）、과양화경매（CAT）화과양화물매（POD）]급병이철함량（MDA）적영향。결과표명：재고 Cu2+농도（600 mg·kg-1）협박하，간작옥미지상부화지하부간중교단작분별제고료20예화36.6예，여단작상비，수착 Cu2+농도승고（100、200、400、600 mg·kg-1），간작모식하옥미지상부동함량분별강저료86.81예、44.57예、22.01예、11.11예，이지하부동함량칙분별제고료78.89예、24.79예、35.29예、13.31예，차차이균체도현저수평。재불동Cu2+농도협박하，옥미협화근중적 SOD、POD、CAT 활성화 MDA 함량균수착 Cu2+농도적증가이제고，부 Cu2+농도체도600 mg·kg-1시옥미체내적 CAT 활성유소하강。간작조건하옥미근적 SOD 활성교단작제고료48.07예~117.27예，간작옥미협적 SOD 활성교단작제고료11.30예~46.90예。불동충식모식대 Cu2+협박조건하적옥미 POD 활성균몰유현저영향。재 Cu2+（0~400 mg·kg-1）협박하，간작옥미협적 CAT 활성교단작균현저제고，분별제고료71.37예、140.40예、229.80예화161.75예，간작옥미근적 CAT 활성여단작무현저차이。간작옥미근적 MDA 함량교단작강저료26.13예~64.53예；재100、200 mg·kg-1 Cu2+협박하，간작옥미협적 MDA 함량교단작강저료0.30예화26.24예，단재400、600 mg·kg-1 Cu2+협박하，간작옥미협적 MDA 함량교단작제고료32.62예화93.51예。종상소술，재일정범위적 Cu2+협박조건하，옥미근화협중적항양화매활성급 MDA 함량균유소제고래유지정상생장，간작모식재 Cu2+협박하대옥미근화협적항양화매계통능기도일정적보호작용。
With industrial development, the area of arable land contaminated by heavy metals is steadily increasing around the world, espe-cially in developing countries. Intercropping has shown the potential to phytoremediate heavy metal polluted soils. A pot experiment was conducted to explore the antioxidant responses of maize to copper(Cu)pollution in red soil under maize-pea intercropping. Five Cu concentrations(0, 100, 200, 400, 600 mg·kg -1)and two cropping patterns(maize monoculture and maize-pea intercropping)were de-signed. Plant biomass, Cu contents, antioxidant enzyme activities(SOD, CAT, POD)and MDA contents of maize were measured. Com-pared with those in the monoculture, the aboveground and underground dry biomass of maize in high Cu2+ concentration(600 mg·kg-1)were increased by 20% and 36.6%, respectively, under the intercropping system. However, Cu contents in the aboveground were reduced by 86.81%, 44.57%, 22.01% and 86.81%, but root Cu increased by 78.89%, 24.79%, 35.29% and 24.79% in 100, 200, 400 mg·kg-1 and 600 mg·kg-1 treatments, respectively, under the intercropping system, compared with the maize monoculture. Significant difference in plant Cu was present between intercropping and monoculture. The SOD, POD, CAT activities and MDA content in leaves and roots of maize all in-creased with increasing Cu2+ concentrations. But Cu2+ addition at 600 mg·kg-1 caused CAT activity decrease. Compared to the monoculture, SOD activity under maize-pea intercropping increased by 48.07%~117.27% in roots and 11.30%~46.90% in leaves. Cropping patterns had no significant effect on POD activity under the same Cu2+ stress. In 0~400 mg·kg-1 Cu stresses, CAT activities in maize leaves were signifi-cantly increased by 71.37%, 140.40%, 229.80% and 161.75% respectively under the intercropping over the monoculture, whereas no sig-nificant difference in the CAT activities in roots was observed between two cropping systems. The intercropping practice reduced MDA con-tents in maize roots by 26.13%~64.53%; while intercropping caused left MDA decline only at 100 mg Cu·kg-1 and 200 mg Cu·kg-1 but in-creased at 400 mg Cu·kg-1 and 600 mg Cu·kg-1, as compared with the monoculture. In summary, intercropping could improve antioxidant enzymatic system in maize roots and leaves and thus alleviate Cu toxicity under Cu2+ stresses.