国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
19期
2670-2671
,共2页
肌钙蛋白I%免疫荧光法%化学发光法
肌鈣蛋白I%免疫熒光法%化學髮光法
기개단백I%면역형광법%화학발광법
troponin I%immunofluorescence%chemiluminescence method
目的：探讨酶联荧光分析（ELFA）法测定肌钙蛋白I（cTnI）的临床应用。方法分别采用ELFA法和化学发光免疫分析（CLIA）法测定血清样品中cTnI的浓度，对ELFA法进行精密度试验、线性试验、回收试验和干扰试验；并对 ELFA 法与CLIA法测定cTnI的结果进行相关性分析。结果 ELFA法测定cTnI浓度，高值（30．00μg/L cTnI）和低值（0．12μg/L cTnI）的批内变异系数（CV）分别为2．81％和1．62％，批间CV分别为5．67％和3．52％。ELFA法检测cTnI的可信检测范围为0．00～30．00μg/L。当总胆红素浓度小于450μmol/L ，血红蛋白浓度小于1．5 g/L ，三酰甘油浓度小于7．0 mmol/L时，ELFA法测定cTnI无干扰。ELFA法与CLIA法测定cTnI浓度具有良好的相关性（r＝0．971）。结论 ELFA法具有较好的精密度、回收率和线性范围，与CLIA法具有较好的相关性，且检测快速简便，适用于急诊检验，能满足临床要求。
目的：探討酶聯熒光分析（ELFA）法測定肌鈣蛋白I（cTnI）的臨床應用。方法分彆採用ELFA法和化學髮光免疫分析（CLIA）法測定血清樣品中cTnI的濃度，對ELFA法進行精密度試驗、線性試驗、迴收試驗和榦擾試驗；併對 ELFA 法與CLIA法測定cTnI的結果進行相關性分析。結果 ELFA法測定cTnI濃度，高值（30．00μg/L cTnI）和低值（0．12μg/L cTnI）的批內變異繫數（CV）分彆為2．81％和1．62％，批間CV分彆為5．67％和3．52％。ELFA法檢測cTnI的可信檢測範圍為0．00～30．00μg/L。噹總膽紅素濃度小于450μmol/L ，血紅蛋白濃度小于1．5 g/L ，三酰甘油濃度小于7．0 mmol/L時，ELFA法測定cTnI無榦擾。ELFA法與CLIA法測定cTnI濃度具有良好的相關性（r＝0．971）。結論 ELFA法具有較好的精密度、迴收率和線性範圍，與CLIA法具有較好的相關性，且檢測快速簡便，適用于急診檢驗，能滿足臨床要求。
목적：탐토매련형광분석（ELFA）법측정기개단백I（cTnI）적림상응용。방법분별채용ELFA법화화학발광면역분석（CLIA）법측정혈청양품중cTnI적농도，대ELFA법진행정밀도시험、선성시험、회수시험화간우시험；병대 ELFA 법여CLIA법측정cTnI적결과진행상관성분석。결과 ELFA법측정cTnI농도，고치（30．00μg/L cTnI）화저치（0．12μg/L cTnI）적비내변이계수（CV）분별위2．81％화1．62％，비간CV분별위5．67％화3．52％。ELFA법검측cTnI적가신검측범위위0．00～30．00μg/L。당총담홍소농도소우450μmol/L ，혈홍단백농도소우1．5 g/L ，삼선감유농도소우7．0 mmol/L시，ELFA법측정cTnI무간우。ELFA법여CLIA법측정cTnI농도구유량호적상관성（r＝0．971）。결론 ELFA법구유교호적정밀도、회수솔화선성범위，여CLIA법구유교호적상관성，차검측쾌속간편，괄용우급진검험，능만족림상요구。
Objective To explore the clinical application of determination of troponin I(cTnI) by enzyme linked fluorescent(EL-FA) method .Methods The concentration of cTnI in serum was detected by chemiluminescence immunoassay(CLIA) method and ELFA method .The precision test ,linear test ,recovery test and interference test were done on ELFA method .The correlation analy-sis was done on the results of cTnI detected by ELFA method and CLIA method .Results Determination of the concentration of cTnI by ELFA method ,the intraassay coefficient of variation(CV) of high(30 .00 μg/L cTnI) and low (0 .12 μg/L cTnI) values were 2 .81% and 1 .62% ,the interassay CV were 5 .67% and 3 .52% .Reliable detection range detection of ELFA method was 0 .00-30 .00 μg/L .When the total bilirubin concentration less than 450μmol/L ,hemoglobin concentration less than 1 .5 g/L ,three glycerol concentration less than 7 .0 mmol/L ,the ELFA method for the determination of cTnI was without interference .Determina-tion of the concentration of cTnI had a good correlation between ELFA method and CLIA method(r=0 .971) .Conclusion The EL-FA method has good precision ,recovery and linear range ,and has a good correlation with the CLIA method ,and the ELFA method is rapid and convenient ,suitable for the emergency inspection ,can satisfy the clinical requirements .
