CAJ | 학술논문

目的：探讨急性白血病患者外周血白细胞介素（IL ）-2、干扰素-γ（IFN-γ）、IL-10水平及血清乳酸脱氢酶（LD H ）活性的变化。方法将65例急性白血病患者分为未缓解组（35例）和缓解组（30例），另选取健康体检者50例为对照组。采用酶联免疫吸附试验（ELISA）检测外周血IL-2、IFN-γ和IL-10的水平，采用全自动生化分析仪检测血清中LDH的活性。结果未缓解组IL-2和IFN-γ水平均明显低于对照组和缓解组（P＜0．05），而IL-10水平则明显高于对照组和缓解组（P＜0．05）；未缓解组血清中LDH活性明显高于对照组和缓解组，差异有统计学意义（P＜0．05）。结论急性白血病患者外周血中IL-2、IFN-γ、IL-10水平及血清中LDH活性会发生明显变化，临床上可以通过检测IL-2、IFN-γ、IL-10水平及LDH活性对急性白血病患者进行早期诊断。
목적：탐토급성백혈병환자외주혈백세포개소（IL ）-2、간우소-γ（IFN-γ）、IL-10수평급혈청유산탈경매（LD H ）활성적변화。방법장65례급성백혈병환자분위미완해조（35례）화완해조（30례），령선취건강체검자50례위대조조。채용매련면역흡부시험（ELISA）검측외주혈IL-2、IFN-γ화IL-10적수평，채용전자동생화분석의검측혈청중LDH적활성。결과미완해조IL-2화IFN-γ수평균명현저우대조조화완해조（P＜0．05），이IL-10수평칙명현고우대조조화완해조（P＜0．05）；미완해조혈청중LDH활성명현고우대조조화완해조，차이유통계학의의（P＜0．05）。결론급성백혈병환자외주혈중IL-2、IFN-γ、IL-10수평급혈청중LDH활성회발생명현변화，림상상가이통과검측IL-2、IFN-γ、IL-10수평급LDH활성대급성백혈병환자진행조기진단。
Objective To study the levels of interleukin(IL)-2 ,interferon-γ(IFN-γ) and IL-10 in peripheral blood and the effec-tive concentration of lactate dehydrogenase(LDH) in serum of patients with acute leukemia .Methods 65 patients with acute leuke-mia were divided into non-remission group(n=35) and remission group(n=30) .50 healthy volunteers were used as control group . The levels of IL-2 ,IFN-γ ,IL-10 in peripheral blood were measured by enzyme linked immunosorbent assay(ELISA) .The effective concentration of LDH was measured by automatic biochemical analyzer .Results The levels of IL-2 ,IFN-γin non-remission group were significantly decreased compared with control group and remission group(P<0 .05) ,but the level of IL-10 in non-remission group was significantly increased compared with control group and remission group(P<0 .05) .The effective concentration of LDH in non-remission group was significantly higher than that in control group or remission group(P<0 .05) .Conclusion The levels of IL-2 ,IFN-γand IL-10 in peripheral blood and the effective concentration of LDH in serum of patients with acute leukemia have changed .Clinical detection of IL-2 ,IFN-γ,IL-10 and LDH may contribute to the early diagnosis of patients with acute leukemia .