CAJ | 학술논문

目的：研究葛根素对人脐静脉内皮细胞增殖、迁移、侵入和血管形成的影响，并探讨其机制。方法四甲基偶氮唑盐（MTT）法测定葛根素对人脐静脉内皮细胞增殖的影响，细胞侵入试验检测葛根素对细胞侵入能力的影响，细胞迁移试验检测葛根素对细胞迁移能力的影响，管道形成试验检测葛根素对细胞血管形成能力的影响，蛋白质印迹法检测磷酸化Akt（p-Akt）、磷酸化一氧化氮合酶（p-eNOS）蛋白水平的表达变化。结果葛根素明显促进了人脐静脉内皮细胞的增殖、迁移、侵入和血管形成能力（P＜0．05），并显著上调p-Akt和p-eNOS蛋白的表达（P＜0．05）。结论葛根素能够促进人脐静脉内皮细胞的增殖、迁移、侵入和血管形成，上调p-Akt和p-eNOS蛋白的表达可能是其机制之一。
목적：연구갈근소대인제정맥내피세포증식、천이、침입화혈관형성적영향，병탐토기궤제。방법사갑기우담서염（MTT）법측정갈근소대인제정맥내피세포증식적영향，세포침입시험검측갈근소대세포침입능력적영향，세포천이시험검측갈근소대세포천이능력적영향，관도형성시험검측갈근소대세포혈관형성능력적영향，단백질인적법검측린산화Akt（p-Akt）、린산화일양화담합매（p-eNOS）단백수평적표체변화。결과갈근소명현촉진료인제정맥내피세포적증식、천이、침입화혈관형성능력（P＜0．05），병현저상조p-Akt화p-eNOS단백적표체（P＜0．05）。결론갈근소능구촉진인제정맥내피세포적증식、천이、침입화혈관형성，상조p-Akt화p-eNOS단백적표체가능시기궤제지일。
Objective To explore the effects of puerarin on proliferation ,invasion ,migration and tube formation in human endo-thelial cells and its possible mechanism .Methods The effect of puerarin on cell proliferation was determined using methyl thiazolyl tetrazolium(MTT) assay .Invasion was evaluated with transwell chamber .Migration was performed by the wound healing method . Endothelial tube formation was performed by tube formation assay .The expressions of phosphorylase Akt(p-Akt) and phosphoryl-ase nitric oxide synthase(p-eNOS) protein were determined by western blot .Results Puerarin promote the proliferation ,invasion , migration and tube formation of human endothelial cells(P<0 .05) .The expression of p-Akt and p-eNOS were increased signifi-cantly(P<0 .05) .Conclusion Puerarin can promote the proliferation ,invasion ,migration and tube formation of human endothelial cells .Up regulation of p-AKT and p-eNOS protein may be one of its mechanisms .